Rabbit anti-Human PSMC3 Polyclonal Antibody | anti-PSMC3 antibody

PSMC3 Antibody

Cat. Num. AAA7005672

• Gene Names: PSMC3; TBP1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: >95%,Protein G purified
• Synonyms: PSMC3; Polyclonal Antibody; PSMC3 Antibody; 26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; Tat-binding protein 1; TBP-1; TBP1; anti-PSMC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 439

• Applicable Applications for anti-PSMC3 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Immunogen: Recombinant human 26S protease regulatory subunit 6A protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7005672 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescence staining of A549 cells with MBS7005672 at 1:530,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-PSMC3 antibody

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, suppresses Tat-mediated transactivation.

NCBI and Uniprot Product Information

• NCBI GI #: 21361144
• NCBI GeneID: 5702
• NCBI Accession #: NP_002795.2
• NCBI GenBank Nucleotide #: NM_002804.4
• UniProt Accession #: P17980; Q3B757; Q3B865; Q53HU5; Q6GPG8; Q6IBS1; Q96HD3; B2R8V1
• Molecular Weight: 49,204 Da
• NCBI Official Full Name: 26S protease regulatory subunit 6A
• NCBI Official Synonym Full Names: proteasome 26S subunit, ATPase 3
• NCBI Official Symbol: PSMC3
• NCBI Official Synonym Symbols: TBP1
• NCBI Protein Information: 26S protease regulatory subunit 6A
• UniProt Protein Name: 26S protease regulatory subunit 6A
• Protein Family: 26S protease regulatory
• UniProt Gene Name: PSMC3
• UniProt Synonym Gene Names: TBP1; TBP-1
• UniProt Entry Name: PRS6A_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]

Uniprot Description

RPT5: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, suppresses Tat-mediated transactivation. Belongs to the AAA ATPase family.

Protein type: Nuclear receptor co-regulator; Proteasome complex; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytosol; membrane; nucleoplasm; nucleus; proteasome complex

Molecular Function: ATP binding; ATPase activity; protein binding; TATA-binding protein binding; transcription coactivator activity; transcription corepressor activity

Biological Process: activation of MAPKK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; apoptosis; axon guidance; blastocyst development; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; epidermal growth factor receptor signaling pathway; ER-associated protein catabolic process; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; gene expression; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; mitotic cell cycle; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; nerve growth factor receptor signaling pathway; polyamine metabolic process; positive regulation of transcriptional preinitiation complex assembly; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; programmed cell death; protein polyubiquitination; Ras protein signal transduction; regulation of amino acid metabolic process; regulation of apoptosis; regulation of mRNA stability; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction