Mouse anti-Dog, Human CD42a Monoclonal Antibody | anti-CD42a antibody

Anti-Hu CD42a PE

Cat. Num. AAA1800662

• Gene Names: GP9; GPIX; CD42a
• Reactivity: Dog, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD42a; Monoclonal Antibody; Anti-Hu CD42a PE; glycoprotein IX platelet; GPIX; GP9; anti-CD42a antibody

• Host: Mouse
• Reactivity: Dog, Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: GR-P
• Specificity: The mouse monoclonal antibody GR-P (also known as GRP-P) recognizes an extracellular epitope of CD42a (glycoprotein 9), a 22 kDa transmembrane protein constitutively expressed on megakaryocytes and platelets.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Sequence Length: 177

• Applicable Applications for anti-CD42a antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Human acute lymphoblastic leukemia cells
• Conjugation: PE
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Separation of human thrombocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42a (GR-P) PE (10 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD42a antibody

CD42a, also known as Glycoprotein 9 (GPIX), composes together with GPIb alpha, GPIb beta and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. Defects in the gene encoding CD42a are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.

NCBI and Uniprot Product Information

• NCBI GI #: 4504077
• NCBI GeneID: 2815
• NCBI Accession #: NP_000165
• NCBI GenBank Nucleotide #: NP_000165.1
• UniProt Accession #: P14770
• NCBI Official Full Name: platelet glycoprotein IX
• NCBI Official Synonym Full Names: glycoprotein IX platelet
• NCBI Official Symbol: GP9
• NCBI Official Synonym Symbols: GPIX; CD42a
• NCBI Protein Information: platelet glycoprotein IX
• UniProt Protein Name: Platelet glycoprotein IX
• UniProt Gene Name: GP9
• UniProt Synonym Gene Names: GP-IX; GPIX

NCBI Description

This gene encodes a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The complete receptor complex includes noncovalent association of the alpha and beta subunits with the protein encoded by this gene and platelet glycoprotein V. Defects in this gene are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency. [provided by RefSeq, Oct 2008]

Uniprot Description

The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.