Mouse CD42a Monoclonal Antibody | anti-CD42a antibody

MOUSE ANTI HUMAN CD42a:FITC

Cat. Num. AAA224730

• Gene Names: GP9; GPIX; CD42a
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD42a; Monoclonal Antibody; MOUSE ANTI HUMAN CD42a:FITC; anti-CD42a antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: FMC-25
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)
• Sequence Length: 177

• Applicable Applications for anti-CD42a antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Preparation
• Histology Positive Control Tissue: Bone marrow; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human peripheral blood platelets stained with Mouse anti Human CD42a:FITC (MBS224730))

Related Product Information for anti-CD42a antibody

Mouse anti Human CD42a antibody, clone FMC-25 recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a single leucine-rich repeat containing N-terminal domain and a single leucine-rich repeat containing C-terminal domain. CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yan et al. 2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearence of giant platelets in the circulation (Johns et al. 2014). Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with anti-phospholipid syndrome (Godeau et al. 1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4504077
• NCBI GeneID: 2815
• NCBI Accession #: NP_000165.1
• NCBI GenBank Nucleotide #: NM_000174.4
• UniProt Accession #: P14770; Q14445; Q8N1D1; Q92525
• Molecular Weight: 19,046 Da
• NCBI Official Full Name: platelet glycoprotein IX
• NCBI Official Synonym Full Names: glycoprotein IX (platelet)
• NCBI Official Symbol: GP9
• NCBI Official Synonym Symbols: GPIX; CD42a
• NCBI Protein Information: platelet glycoprotein IX; glycoprotein 9
• UniProt Protein Name: Platelet glycoprotein IX
• UniProt Gene Name: GP9
• UniProt Synonym Gene Names: GP-IX; GPIX
• UniProt Entry Name: GPIX_HUMAN

NCBI Description

This gene encodes a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The complete receptor complex includes noncovalent association of the alpha and beta subunits with the protein encoded by this gene and platelet glycoprotein V. Defects in this gene are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency. [provided by RefSeq, Oct 2008]

Uniprot Description

GPIX: The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib. Defects in GP9 are a cause of Bernard-Soulier syndrome (BSS); also known as giant platelet disease (GPD). BSS patients have unusually large platelets and have a clinical bleeding tendency.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: integral to plasma membrane; plasma membrane

Biological Process: platelet activation; blood coagulation; cell adhesion; blood coagulation, intrinsic pathway

Disease: Bernard-soulier Syndrome