Typical Testing Data/Standard Curve (for reference only)
Human Mannose 1 phosphate guanyltransferase beta (GMPPB) ELISA Kit | GMPPB elisa kit
Human Mannose 1 phosphate guanyltransferase beta (GMPPB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GMPPB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GMPPB antibody and an GMPPB-HRP conjugate. The assay sample and buffer are incubated together with GMPPB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GMPPB concentration since GMPPB from samples and GMPPB-HRP conjugate compete for the anti-GMPPB antibody binding site. Since the number of sites is limited, as more sites are occupied by GMPPB from the sample, fewer sites are left to bind GMPPB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GMPPB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is thought to encode a GDP-mannose pyrophosphorylase. The encoded protein catalyzes the conversion of mannose-1-phosphate and GTP to GDP-mannose, a reaction involved in the production of N-linked oligosaccharides. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
GMPPB: This gene is thought to encode a GDP-mannose pyrophosphorylase. The encoded protein catalyzes the conversion of mannose-1-phosphate and GTP to GDP-mannose, a reaction involved in the production of N-linked oligosaccharides. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2009]
Protein type: Motility/polarity/chemotaxis; EC 2.7.7.13; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: mitochondrion; cytoplasm
Molecular Function: GTP binding; mannose-1-phosphate guanylyltransferase activity
Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; GDP-mannose biosynthetic process; post-translational protein modification
Disease: Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 14; Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 14; Muscular Dystrophy-dystroglycanopathy (congenital With Mental Retardation), Type B, 14
Research Articles on GMPPB
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Product Notes
The Human GMPPB gmppb (Catalog #AAA7241128) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7241128 ELISA Kit recognizes Human GMPPB. It is sometimes possible for the material contained within the vial of "Mannose 1 phosphate guanyltransferase beta (GMPPB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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