Rat anti-Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

Anti-Mouse CD4 FITC (Clone CT-CD4) (rat IgG2a)

Cat. Num. AAA520110

• Gene Names: CD4; CD4mut
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Synonyms: CD4; Monoclonal Antibody; Anti-Mouse CD4 FITC (Clone CT-CD4) (rat IgG2a); CD4 FITC (Clone CT-CD4) (rat IgG2a); FITC Anti-Mouse CD4 (L3/T4) Monoclonal Antibody; anti-CD4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone Number: CT-CD4
• Specificity: CD4 (L3/T4; Ly4) (Mouse CD4)
• Form/Format: FITC
• Concentration: Antibody Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 71

• Applicable Applications for anti-CD4 antibody: Immunohistochemistry (IHC)
• Presentation: 100 ug or 300 ug FITC conjugated Ig buffered in PBS, 0.1% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
• Preparation and Storage: Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles and prolonged exposure to light. Check label for expiry date.

Testing Data

Related Product Information for anti-CD4 antibody

CT-CD4 monolconal antibody (mAb) recognizes mouse CD4 (L3T4) which is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells including Th cells1,2. Mouse CD4 has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors2,3. As a coreceptor in the TCR complex, CD4 is involved in T cell activation through interaction with MHC class II on APC's and in signal transduction via protein tyrosine kinase lck1. This antibody is suitable for use in flow cytometry and is reported to work in immunohistochemistry on acetone fixed frozen sections.

NCBI and Uniprot Product Information

• NCBI GI #: 3947700
• NCBI GeneID: 920
• UniProt Accession #: Q13969; P01730
• Molecular Weight: 7,844 Da
• NCBI Official Full Name: CD4
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: CD4 protein
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Synonym Gene Names: CD4 receptor
• UniProt Entry Name: Q13969_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; signal transduction; induction by virus of cell-cell fusion in host; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation

Disease: Okt4 Epitope Deficiency