Rat Troponin I Type 2, Fast Skeletal (TNNI2) ELISA Kit | TNNI2 elisa kit

Rat Troponin I Type 2, Fast Skeletal (TNNI2) ELISA Kit

Cat. Num. AAA2704986

• Gene Names: TNNT3; TNTF
• Reactivity: Rat
• Synonyms: Troponin I Type 2; Fast Skeletal (TNNI2); Rat Troponin I Type 2; Fast Skeletal (TNNI2) ELISA Kit; AMCD2B; DA2B; FSSV; Troponin I Fast Twitch 2; Troponin I; fast-twitch isoform; TNNI2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Troponin I Type 2, Fast Skeletal (TNNI2).; No significant cross-reactivity or interference between Troponin I Type 2, Fast Skeletal (TNNI2) and analogues was observed.
• Sequence Length: 256

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 2.34-150ng/mL
• Sensitivity: < 0.65ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin I Type 2, Fast Skeletal (TNNI2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin I Type 2, Fast Skeletal (TNNI2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNNI2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 2, Fast Skeletal (TNNI2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin I Type 2, Fast Skeletal (TNNI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 2, Fast Skeletal (TNNI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 2, Fast Skeletal (TNNI2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 112789538
• NCBI GeneID: 7140
• NCBI Accession #: NP_001036246.1
• NCBI GenBank Nucleotide #: NM_001042781.2
• UniProt Accession #: P45378; Q12975; Q12976; Q12977; Q12978; Q17RG9; A8MQ76; A8MSW1; B3KPX3; B7WP64; B7ZL26; B7ZVV9
• Molecular Weight: 29,735 Da
• NCBI Official Full Name: troponin T, fast skeletal muscle isoform 2
• NCBI Official Synonym Full Names: troponin T3, fast skeletal type
• NCBI Official Symbol: TNNT3
• NCBI Official Synonym Symbols: TNTF
• NCBI Protein Information: troponin T, fast skeletal muscle
• UniProt Protein Name: Troponin T, fast skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT3
• UniProt Synonym Gene Names: TnTf; fTnT

NCBI Description

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]

Uniprot Description

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.