Mouse anti-Human CD357 Monoclonal Antibody | anti-CD357 antibody

anti-human CD357(GITR)-FITC

Cat. Num. AAA666414

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: CD357; Monoclonal Antibody; anti-human CD357(GITR)-FITC; Monoclonal anti-human CD357 (GITR; AITR)/FITC (IgM isotype); anti-CD357 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgMk
• Clone Number: ANC7D6-1B7
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2 ml (varies by lot)

• Applicable Applications for anti-CD357 antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: 1:50 (or use 1.6 ul of concentrated reagent per 5 x 105-cell test)
• Performance: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-GITR/FITC at a 1:50 dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated with a 10-fold excess of unlabeled anti-GITR mAb.
• Conjugation: FITC
• Production: Antibody from (low FBS containing) tissue culture supernatant was purified by size exclusion chromatographand reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at 0.5 mg/ml with a Fluorescein/IgM molar ratio of 35.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant human GITR, PHA stimulated human PBL
• Preparation and Storage: Store at 2 - 5 degree C. Freeze thawing not recommended. Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD357 antibody

Information: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (CD357, GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in anti-parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3). Clone ANC7D6-1E7 binds to human CD357 in EIA and on stimulated cell surface in FACS. Pre incubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.

References

1) Gurney AL, et al. (1999) Curr Biol 9(4): 215-218. 2) Ji HB, et al. (2004) J Immunol 172(10): 5823-5827. 3) Kim WJ, WH Lee, et al. (2006) Immunology 119(3): 44-9. 4) Furze RC, Selkirk ME, et al. (2006) Microbes Infect 8(12-13): 2803-10. 5) Kohm AP, SD Miller, et al. (2004) J Immunol 172: 4686-4690. 6) McHugh, R.S. et al. (2002) Immunity 16:311. 7) Shin, H.H. et al. (2002) FEBS Lett. 514:275. 8). Shimizu, J. et al. (2002) Nature Immunol. 3:135.