Rabbit anti-Human, Mouse AMH Polyclonal Antibody | anti-AMH antibody

AMH, ID (AMH, MIF, Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance) (APC)

Cat. Num. AAA6273225

• Gene Names: AMH; MIF; MIS
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: AMH; Polyclonal Antibody; ID (AMH; MIF; Muellerian-inhibiting factor; Anti-Muellerian hormone; Muellerian-inhibiting substance) (APC); Muellerian-inhibiting substance; anti-AMH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AMH. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AMH antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 424-451 in the central region of human AMH, Swiss/UniProt Accession: P03971.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6000643. AMH (arrow) was detected usingMBS6000643.)

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6000643. AMH (arrow) was detected usingMBS6000643.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded prostate carcinoma using MBS6000643 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of 293 cells (right histogram) using MBS6000643 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-AMH antibody

Antibodies; Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 157266298
• NCBI GeneID: 268
• NCBI Accession #: NP_000470.2
• NCBI GenBank Nucleotide #: NM_000479.3
• UniProt Accession #: P03971; O75246; Q6GTN3
• Molecular Weight: 59,195 Da
• NCBI Official Full Name: muellerian-inhibiting factor
• NCBI Official Synonym Full Names: anti-Mullerian hormone
• NCBI Official Symbol: AMH
• NCBI Official Synonym Symbols: MIF; MIS
• NCBI Protein Information: muellerian-inhibiting factor; anti-Muellerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance; muellerian-inhibiting substance
• UniProt Protein Name: Muellerian-inhibiting factor
• Protein Family: Muellerian-inhibiting factor
• UniProt Gene Name: AMH
• UniProt Synonym Gene Names: MIF; AMH; MIS
• UniProt Entry Name: MIS_HUMAN

NCBI Description

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; hormone activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: response to drug; sex determination; Mullerian duct regression; cell-cell signaling; gonadal mesoderm development; preantral ovarian follicle growth; sex differentiation; response to organic cyclic substance; urogenital system development; activation of NF-kappaB transcription factor; aging

Disease: Persistent Mullerian Duct Syndrome, Types I And Ii