Hamster CD31 Monoclonal Antibody | anti-CD31 antibody
HAMSTER ANTI MOUSE CD31:Preservative Free
Gene Names
Pecam1; Cd31; Pecam; C85791; PECAM-1
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified IgG prepared by affinity chromatography on Protein G ascites
Synonyms
CD31; Monoclonal Antibody; HAMSTER ANTI MOUSE CD31:Preservative Free; anti-CD31 antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
2H8
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G ascites
Form/Format
Preservative Free
Purified IgG - liquid; Phosphate buffered saline!
Purified IgG - liquid; Phosphate buffered saline!
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Sequence Length
697
Applicable Applications for anti-CD31 antibody
Flow cytometry (FC), Functional Assays (FN), Immunofluorescence (IF)
Application Notes
Flow Cytometry: Use 5ul of the suggested working dilution to label 100ul of whole blood.
Flow Cytometry: Maximum Dilution: 0.1ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Maximum Dilution: 0.1ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Perservative Stabilisers
None present, Sterile filtered.
Immunogen
D10.G4.1 Cells
Target Species
Mouse
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Fusion Partners
Splenic lymphocytes from an immunized Armenian hamster were fused with cells from the SP2/0 murine myeloma
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-CD31 antibody
Hamster anti Mouse CD31 monoclonal antibody, clone 2H8 recognizes murine CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line (Bogen et al. 1992). Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994). CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis (Stevens et al. 2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
platelet endothelial cell adhesion molecule isoform 2
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
Pecam1
NCBI Official Synonym Symbols
Cd31; Pecam; C85791; PECAM-1
NCBI Protein Information
platelet endothelial cell adhesion molecule
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
Pecam1
UniProt Synonym Gene Names
Pecam; Pecam-1; PECAM-1
UniProt Entry Name
PECA1_MOUSE
Uniprot Description
PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
Protein type: Cell adhesion; Membrane protein, integral
Cellular Component: extracellular space; smooth muscle contractile fiber; cell surface; membrane; integral to membrane; plasma membrane; intracellular; cell junction; external side of plasma membrane; lipid raft
Molecular Function: protein binding
Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; wound healing; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of actin filament polymerization; diapedesis; positive regulation of leukocyte migration; cell adhesion; regulation of cell migration; Rho protein signal transduction; phagocytosis
Research Articles on CD31
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Product Notes
The CD31 pecam1 (Catalog #AAA210144) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC), Functional Assays (FN), Immunofluorescence (IF). Flow Cytometry: Use 5ul of the suggested working dilution to label 100ul of whole blood. Flow Cytometry: Maximum Dilution: 0.1ug/ml Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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