Mouse PECAM-1/CD31 ELISA Kit | PECAM1 elisa kit

Mouse PECAM-1/CD31 PicoKine ELISA Kit

Cat. Num. AAA175812

• Gene Names: Pecam1; Cd31; Pecam; C85791; PECAM-1
• Reactivity: Mouse
• Synonyms: PECAM-1/CD31; Mouse PECAM-1/CD31 PicoKine ELISA Kit; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1f; CD31; CD31 antigen; EndoCAM; GPIIA; HEC7; Leukocyte surface protein; PECA1; PECA1_HUMAN; PECAM 1; PECAM-1; Pecam1; Platelet/endothelial cell adhesion molecule; PECAM1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse PECAM-1
• Sequence Length: 727

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse PECAM-1/CD31 PicoKine ELISA Kit standard curve )

Related Product Information for PECAM1 elisa kit

Principle of the assay: mouse PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PECAM-1 has been precoated onto 96-well plates. Standards(NSO, E18-K590) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PECAM-1 amount of sample captured in plate.
Background: PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kDmember of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of theimmunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitorymotifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is theproduct of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 585658
• NCBI GeneID: 18613
• UniProt Accession #: Q08481; Q3TES6; Q922E0; B1ARB1; B1ARB2
• Molecular Weight: 69,823 Da
• NCBI Official Full Name: Platelet endothelial cell adhesion molecule
• NCBI Official Synonym Full Names: platelet/endothelial cell adhesion molecule 1
• NCBI Official Symbol: Pecam1
• NCBI Official Synonym Symbols: Cd31; Pecam; C85791; PECAM-1
• NCBI Protein Information: platelet endothelial cell adhesion molecule; PECAM-1/CD31
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• Protein Family: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: Pecam1
• UniProt Synonym Gene Names: Pecam; Pecam-1; PECAM-1
• UniProt Entry Name: PECA1_MOUSE

Uniprot Description

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: extracellular space; cell surface; smooth muscle contractile fiber; membrane; integral to membrane; plasma membrane; intracellular; cell junction; lipid raft; external side of plasma membrane

Molecular Function: protein binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; wound healing; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of actin filament polymerization; positive regulation of leukocyte migration; diapedesis; cell adhesion; regulation of cell migration; phagocytosis; Rho protein signal transduction