Mouse CD31 Monoclonal Antibody | anti-CD31 antibody

MOUSE ANTI PIG CD31

Cat. Num. AAA224382

• Gene Names: PECAM1; CD31; PECAM-1
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
• Synonyms: CD31; Monoclonal Antibody; MOUSE ANTI PIG CD31; anti-CD31 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LCI-4
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 740

• Applicable Applications for anti-CD31 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Pig
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD31:FITC (MBS210763))

Related Product Information for anti-CD31 antibody

Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmambrane region as demonstrated by human CD31 domain deletion mutant protein binding studies (Nasu et al.1999). Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999).

NCBI and Uniprot Product Information

• NCBI GI #: 47522670
• NCBI GeneID: 396941
• NCBI Accession #: NP_999072.1
• NCBI GenBank Nucleotide #: NM_213907.1
• UniProt Accession #: Q95242
• Molecular Weight: 82,379 Da
• NCBI Official Full Name: platelet endothelial cell adhesion molecule
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECAM-1
• NCBI Protein Information: platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule (CD31 antigen); type I transmembrane endothelial adhesion molecule
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1
• UniProt Entry Name: PECA1_PIG

Uniprot Description

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-692 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation. Induces susceptibility to atherosclerosis ().