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SDS-Page

Mercaptopyruvate Sulfurtransferase Recombinant Protein | MPST recombinant protein

Recombinant Mercaptopyruvate Sulfurtransferase (MPST)

Gene Names
MPST; MST; TST2; TUM1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Mercaptopyruvate Sulfurtransferase; Recombinant Mercaptopyruvate Sulfurtransferase (MPST); MPST recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MAAPQLFRAL VSAQWVAEAL KAPRSSQPLK LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDRCSDHTS PYDHMLPNAT HFADYAGSLG VSAATHVVIY DGSDQGPYSA PRVWWMFRAF GHHSVSLLDG GFRHWLNQNL PISSGKSHSE PAEFSAQLDP SFIKTHEDIL ENLDARRFQV VDARAAGRFQ GTQPEPRDGI EPGHIPGSVN IPFTEFLTNE GLEKSPEEIK RLFKEKKVDL SKPLVATCGS GVTACHVVLG AFLCGKSDVP VYDGSWVEWY MRAQPEHIIS EGRGKTQ
Sequence Length
297
Applicable Applications for MPST recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
36.7kDa
Accurate Molecular Mass (KD)
37kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag
Organism Species
Mus musculus (Mouse)
Fragment
Met1~Gln297
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
35,250 Da
NCBI Official Full Name
Mercaptopyruvate sulfurtransferase
NCBI Official Synonym Full Names
mercaptopyruvate sulfurtransferase
NCBI Official Symbol
MPST
NCBI Official Synonym Symbols
MST; TST2; TUM1
NCBI Protein Information
3-mercaptopyruvate sulfurtransferase
UniProt Protein Name
3-mercaptopyruvate sulfurtransferase
UniProt Gene Name
MPST
UniProt Synonym Gene Names
TST2; MST
UniProt Entry Name
THTM_HUMAN

NCBI Description

This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MPST: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis.

Protein type: Amino Acid Metabolism - cysteine and methionine; Mitochondrial; Transferase; EC 2.8.1.2

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: neuron projection; mitochondrial inner membrane; synapse; cell junction

Molecular Function: 3-mercaptopyruvate sulfurtransferase activity; thiosulfate sulfurtransferase activity

Biological Process: cyanate catabolic process; response to toxin

Research Articles on MPST

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Product Notes

The MPST mpst (Catalog #AAA2029969) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mercaptopyruvate Sulfurtransferase can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the MPST mpst for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MAAPQLFRA L VSAQWVAEAL KAPRSSQPLK LLDASWYLPK LGRDARREFE ERHIPGAAFF DIDRCSDHTS PYDHMLPNAT HFADYAGSLG VSAATHVVIY DGSDQGPYSA PRVWWMFRAF GHHSVSLLDG GFRHWLNQNL PISSGKSHSE PAEFSAQLDP SFIKTHEDIL ENLDARRFQV VDARAAGRFQ GTQPEPRDGI EPGHIPGSVN IPFTEFLTNE GLEKSPEEIK RLFKEKKVDL SKPLVATCGS GVTACHVVLG AFLCGKSDVP VYDGSWVEWY MRAQPEHIIS EGRGKTQ. It is sometimes possible for the material contained within the vial of "Mercaptopyruvate Sulfurtransferase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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