Rabbit anti-Human, Mouse CD3 zeta Polyclonal Antibody | anti-CD3 zeta antibody

CD3 zeta (Phospho-Tyr142) Antibody

Cat. Num. AAA9408605

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: CD3 zeta; Polyclonal Antibody; CD3 zeta (Phospho-Tyr142) Antibody; T3Z; CD3-zeta; TCRZ; T-cell receptor T3 zeta chain; anti-CD3 zeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of CD3 zeta only when phosphorylated at tyrosine 142.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 163

• Applicable Applications for anti-CD3 zeta antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 142 (G-L-Y(p)-Q-G) derived from Human CD3 zeta.
• Modification: Phospho-Tyr142
• Target Name: CD3 zeta
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 zeta (Phospho-Tyr142) Antibody. The lane on the left is treated with antigen-specific peptide.)

Related Product Information for anti-CD3 zeta antibody

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029. Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423. Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330. Hisashi Arase, J. Immunol., Jan 2001; 166: 21.

Product Categories/Family for anti-CD3 zeta antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557431
• NCBI GeneID: 919
• NCBI Accession #: NP_000725.1
• NCBI GenBank Nucleotide #: NM_000734.3
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• Molecular Weight: – Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 zeta chain isoform 2
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ
• UniProt Entry Name: CD3Z_HUMAN

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: T cell receptor complex; cytoplasm; integral to membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity

Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus

Disease: Immunodeficiency 25