Mouse CD3 Monoclonal Antibody | anti-CD3 antibody

MOUSE ANTI PIG CD3: Low Endotoxin

Cat. Num. AAA224784

• Gene Names: CD3E; CD3
• Reactivity: Does not react with: Bovine, Goat, Horse, Human, Sheep
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot, Functional Assay
• Synonyms: CD3; Monoclonal Antibody; MOUSE ANTI PIG CD3: Low Endotoxin; anti-CD3 antibody

• Host: Mouse
• Reactivity: Does not react with: Bovine, Goat, Horse, Human, Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: PPT3
• Specificity: CD3 EPSILON
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 196

• Applicable Applications for anti-CD3 antibody: Flow Cytometry, Immunohistology-Frozen, Immunoprecipitaion, Western Blotting, Functional Assays.
• Application Notes: Flow Cytometry: 1/50-1/200; ; Use 10 uL of the suggested working dilution to label 1x10⁶ cells in 100uL
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Target Species: Pig
• Preservative Stabiizers: None present
• Endotoxin Level: <0.01 EU/ug
• Immunogen: Porcine PBMCs
• Carrier Free: yes
• Preparation and Storage: Store at -20°C only. ; Storage in frost-free freezers is not recommended.; This product should be stored undiluted. ; Avoid repeated freezing and thawing as this may denature the antibody. ; Should this product contain a precipitate we recommend microcentrifugation before use. ; ; Shelf Life: 18 months from date of despatch

Related Product Information for anti-CD3 antibody

Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3epsilon, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3epsilon designated as CD3c (Pescovitz, M.D., et al. 1998).CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilongamma, epsilondelta, zetazeta). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells. Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate alpha/beta T-cells (Kirkham P.A., et al. 1996). Epitope mapping studies have demonstrated that clone PPT3 recognises an alternative epitope to clone PPT6 (Yang, H. et al. 1996; Pescovitz, M.D., et al. 1998). Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).

NCBI and Uniprot Product Information

• NCBI GI #: 78099944
• NCBI GeneID: 397455
• UniProt Accession #: Q7YRN2
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: CD3
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3E
• UniProt Entry Name: CD3E_PIG

Uniprot Description

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.