Flow Cytometry (FC/FACS)
(Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-mouse CD3 (145-2C11) purified antibody (low endotoxin, concentration in sample 4 ug/ml) DAR APC.)
anti-Mouse CD3 Monoclonal Antibody | anti-CD3E antibody
Anti-Ms CD3 Purified Low Endotoxin
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein-G Affinity Chromatography
Synonyms
CD3; Monoclonal Antibody; Anti-Ms CD3 Purified Low Endotoxin; CD3 antigen; epsilon polypeptide; CD3E; T3E; TCRE; anti-CD3E antibody
Host
Armenian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG kappa
Clone Number
145-2C11
Specificity
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
Purity/Purification
Purified by Protein-G Affinity Chromatography
Form/Format
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Concentration
1 mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-CD3E antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), Functional
Application Notes
FC/FACS: QC tested
Immunogen
Mouse BM10-37 cytotoxic T lymphocytes
Preparation and Storage
Store at 2-8 degree C.
Flow Cytometry (FC/FACS)
(Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-mouse CD3 (145-2C11) purified antibody (low endotoxin, concentration in sample 4 ug/ml) DAR APC.)
Flow Cytometry (FC/FACS)
(Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-mouse CD3 (145-2C11) purified antibody (low endotoxin, concentration in sample 4 ug/ml) DAR APC.)
Related Product Information for anti-CD3E antibody
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3 antigen, epsilon polypeptide
NCBI Official Symbol
Cd3e
NCBI Official Synonym Symbols
CD3; T3e; AI504783; CD3epsilon
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
Protein Family
UniProt Gene Name
Cd3e
UniProt Entry Name
CD3E_MOUSE
Research Articles on CD3E
Similar Products
Product Notes
The CD3E cd3e (Catalog #AAA1800040) is an Antibody produced from Armenian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ms CD3 Purified Low Endotoxin reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC) Frozen, Immunocytochemistry (ICC), Functional. FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD3E cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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