Hamster anti-Mouse CD3 Epsilon Monoclonal Antibody | anti-CD3 antibody

Anti-Mouse CD3 Epsilon Monoclonal Antibody (PE Conjugated)

Cat. Num. AAA2556535

• Gene Names: Cd3e; CD3; T3e; AI504783; CD3epsilon
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3 Epsilon; Monoclonal Antibody; Anti-Mouse CD3 Epsilon Monoclonal Antibody (PE Conjugated); T-cell surface glycoprotein CD3 epsilon chain; CD3E; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; T3E; anti-CD3 antibody

• Host: Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 145-2C11
• Form/Format: Liquid; PBS with 0.05% Proclin300, 1% BSA
• Sequence Length: 189

• Applicable Applications for anti-CD3 antibody: Flow Cytometry (FC/FACS)
• Conjugation: PE
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse CD3epsilon Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD3 antibody

CD3epsilon is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3epsilon forms a TCR complex by associating with the CD3delta, gamma and zeta chains, as well as the TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

NCBI and Uniprot Product Information

• NCBI GI #: 6680886
• NCBI GeneID: 12501
• NCBI Accession #: NP_031674.1
• NCBI GenBank Nucleotide #: NM_007648.5
• UniProt Accession #: P22646
• Molecular Weight: 21,393 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3 antigen, epsilon polypeptide
• NCBI Official Symbol: Cd3e
• NCBI Official Synonym Symbols: CD3; T3e; AI504783; CD3epsilon
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: Cd3e

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.