Rabbit ATP citrate lyase Polyclonal Antibody | anti-ATP antibody

ATP citrate lyase Antibody

Cat. Num. AAA9613376

• Gene Names: ACLY; ACL; ATPCL; CLATP
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: ATP citrate lyase; Polyclonal Antibody; ATP citrate lyase Antibody; ACL; Acly; ACLY_HUMAN; ATP citrate (pro-S) lyase; ATP citrate synthase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPcitrate synthase; ATPCL; Citrate cleavage enzyme; CLATP; OTTHUMP00000164773; anti-ATP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (91%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ATP citrate lyase Antibody detects endogenous levels of total ATP citrate lyase.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ATP antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ATP citrate lyase, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).
• Subunit Structure: Homotetramer.
• Similarity: In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
• Subcellular Location: Cytoplasm>Cytosol.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using ATP citrate lyase Antibody.Lane 1: NIH-3T3 cells, blocked with antigen-specific peptides,Lane 2: NIH-3T3 cells,Lane 3: A2780 cells.)

Western Blot (WB)

(Western blot analysis of ATP citrate lyase in lysates of COS7 , using ATP citrate lyase Antibody(AF4668).)

Related Product Information for anti-ATP antibody

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

NCBI and Uniprot Product Information

• NCBI GI #: 38569421
• NCBI GeneID: 47
• NCBI Accession #: NP_001087.2
• NCBI GenBank Nucleotide #: NM_001096.2
• UniProt Accession #: P53396; Q13037; Q9BRL0; B4DIM0
• Molecular Weight: 120,839 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 1
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: ACLY
• NCBI Official Synonym Symbols: ACL; ATPCL; CLATP
• NCBI Protein Information: ATP-citrate synthase; ATP citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
• UniProt Protein Name: ATP-citrate synthase
• UniProt Gene Name: ACLY
• UniProt Synonym Gene Names: ACL
• UniProt Entry Name: ACLY_HUMAN

NCBI Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.3.8; Transferase; Carbohydrate Metabolism - citrate (TCA) cycle; Lyase

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; membrane; mitochondrion; citrate lyase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; ATP citrate synthase activity; metal ion binding; succinate-CoA ligase (ADP-forming) activity; cofactor binding; ATP binding

Biological Process: coenzyme A metabolic process; lipid biosynthetic process; positive regulation of cellular metabolic process; energy reserve metabolic process; citrate metabolic process; cellular carbohydrate metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process