Rat anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody

RAT ANTI HUMAN CD28:FITC

Cat. Num. AAA211074

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD28; Monoclonal Antibody; RAT ANTI HUMAN CD28:FITC; anti-CD28 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: YTH913.12
• Specificity: CD28; This item recognises the human CD28 cell surface antigen, a 44kD glycoprotein expressed by T lymphocytes.; ; It has been reported that this clone enhances the MLR response Clone YTH913.12 recognizes an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2 For functional studies,
• Form/Format: FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 123

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FACS)
• Application Notes: Flow Cytometry: Max Dilution: Neat; Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul human whole blood.
• Immunogen: Human peripheral blood T-cells
• Fusion Partners: Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Preservative Stabilizers: 0.09% Sodium Azide; 1% Bovine Serum Albumin
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at +4 degree C or at -20 degree C if preferred.; ; This product should be stored undiluted.; ; Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.; ; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of dispatch.

Testing Data #1

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin (MBS210509))

Testing Data #4

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28 (MBS213120))

Testing Data #5

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Alexa FluorR 647 (MBS2141859))

Testing Data #7

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28:FITC (MBS211074))

Testing Data #8

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28:RPE (MBS213865))

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN