Flow Cytometry (FC/FACS)
(Con-A stimulated C57BL/6 splenocytes (3 days) are stained with Anti-Mouse CD279/PD-1 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Rat anti-Mouse CD279/PD-1 Monoclonal Antibody | anti-CD279/PD-1 antibody
Anti-Mouse CD279/PD-1 Monoclonal Antibody (PE Conjugated) [29F.1A12]
Gene Names
Pdcd1; PD-1; Pdc1; Ly101
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD279/PD-1; Monoclonal Antibody; Anti-Mouse CD279/PD-1 Monoclonal Antibody (PE Conjugated) [29F.1A12]; PD-1; Programmed Death-1; anti-CD279/PD-1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
29F.1A12
Form/Format
PBS with 0.05% Proclin300, 1% BSA
Concentration
0.5 mg/mL (varies by lot)
Sequence Length
288
Applicable Applications for anti-CD279/PD-1 antibody
Flow Cytometry (FC/FACS)
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Conjugation
PE
Preparation and Storage
Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.
Flow Cytometry (FC/FACS)
(Con-A stimulated C57BL/6 splenocytes (3 days) are stained with Anti-Mouse CD279/PD-1 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Flow Cytometry (FC/FACS)
(Con-A stimulated C57BL/6 splenocytes (3 days) are stained with Anti-Mouse CD279/PD-1 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)
Related Product Information for anti-CD279/PD-1 antibody
CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
Pdcd1
NCBI Official Synonym Symbols
PD-1; Pdc1; Ly101
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
Pdcd1
UniProt Synonym Gene Names
Pd1; Protein PD-1; mPD-1
UniProt Entry Name
PDCD1_MOUSE
Uniprot Description
PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.
Protein type: Apoptosis; Membrane protein, integral
Cellular Component: external side of plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: negative regulation of apoptosis; negative regulation of tolerance induction; positive regulation of apoptosis
Research Articles on CD279/PD-1
Similar Products
Product Notes
The CD279/PD-1 pdcd1 (Catalog #AAA2558953) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD279/PD-1 Monoclonal Antibody (PE Conjugated) [29F.1A12] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD279/PD-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 2 ug per 106 cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Researchers should empirically determine the suitability of the CD279/PD-1 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD279/PD-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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