Platelet Derived Growth Factor Subunit A (PDGFA) Recombinant Protein | PDGFA recombinant protein

Recombinant Platelet Derived Growth Factor Subunit A (PDGFA)

Cat. Num. AAA2097546

• Gene Names: PDGFA; PDGF1; PDGF-A
• Reactivity: Rat
• Applications: SDS-Page, Western Blot
• Purity: > 97%
• Synonyms: Platelet Derived Growth Factor Subunit A (PDGFA); Recombinant Platelet Derived Growth Factor Subunit A (PDGFA); PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide; PDGFA recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 211

• Applicable Applications for PDGFA recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Expression System: Prokaryotic expression
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Fragment: Glu90~Glu190
• Subcellular Location: Secreted
• Isoelectric Point: 9.1

Sequence Information

SDS-Page

Product Categories/Family for PDGFA recombinant protein

Cytokine; Tumor immunity; Infection immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 77695917
• NCBI GeneID: 5154
• NCBI Accession #: NP_002598.4
• NCBI GenBank Nucleotide #: NM_002607.5
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: Predicted Molecular Weight: 15.2kDa; Accurate Molecular Weight: 15kDa
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet derived growth factor subunit A
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; Golgi membrane; microvillus

Molecular Function: collagen binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: actin cytoskeleton organization; alveolus development; angiogenesis; cell activation; cell projection assembly; cell-cell signaling; extracellular matrix organization; hair follicle development; MAPK cascade; negative chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; organ morphogenesis; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of MAPK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling; regulation of actin cytoskeleton organization; regulation of peptidyl-tyrosine phosphorylation; regulation of phosphatidylinositol 3-kinase signaling; regulation of smooth muscle cell migration; response to wounding; skin development; wound healing