anti-Mouse CD27 Monoclonal Antibody | anti-CD27 antibody

American Hamster anti CD27

Cat. Num. AAA570194

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Immunoprecipitation
• Synonyms: CD27; Monoclonal Antibody; American Hamster anti CD27; anti-CD27 antibody

• Host: American Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: LG.8A6
• Specificity: LG.3A10 and LG.8A6 see a different epitope. A combination can therefore be developed into a double determinant ELISA to detect soluble Mouse CD27. LG.3A10 cross-react with Human CD27, LG.8A6 does not.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-CD27 antibody: ELISA (EIA), Flow Cytometry (FC), Immunoprecipitation (IP)
• Application Notes: LG.8A6 is useful for flow cytometry and, immunoprecipitation. Optimal antibody dilution should be determined by titRation. In combination with LG.3A10, it is possible to develop a double determinant ELISA to detect soluble Mouse CD27.
• Source Note: mAbs to murine CD27 were geneRated injecting a mixture of the mCD27tag expresssing clones AR0524 and AR0530; Hamster spleen cells and SP2/0 murine myeloma cells were fused.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-CD27 antibody

CD27 (Cluster of Differentiation 247) is a tumor necrosis factor receptor. A tumor necrosis factor receptor (TNFR), or death receptor, is a trimeric cytokine receptor that binds tumor necrosis factors (TNF).. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for geneRation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. The immune system has several effector mechanisms at its disposal to free the host of pathogens. Because these effector mechanisms can also damage the host, immune responses should be tightly controlled. TNFR family members and their respective TNF related ligands have been impliCated in the regulation of survival, prolifeRation, differentiation, and migRation during adaptive immune responses. CD27 is a lymphoid cell-specific member of the TNFR family. Human and murine T, B, and NK cells can express CD27 In Humans, CD27 expression distinguishes between naive and effector/memory stages of peripheral B and T lymphocytes. On B cells, CD27 is only found after B cell receptor-induced activation. CD27-expressing B cells found in Human peripheral blood all have undergone somatic hypermutation and therefore CD27 is considered a marker for memory B cells in Humans. Resting T cells constitutively express CD27, while differentiation into effector T cells is accompanied by loss of CD27 expression.

Product Categories/Family for anti-CD27 antibody

CD markers; Immunology

References

Gravestein, L.A., Nieland, J.D., Kruisbeek, A.M. and Borst, J. Int. Immunol. 7: 551-557, 1995. Gravestein, L., van Ewijk, W., Ossendorp, F. and Borst, J. J. Exp. Med. 184: 675-685, 1996. Hendriks, J., Gravestein, L.A., Tesselaar, K., van Lier, R.A.W., Schumacher, T.N.M., and Borst, J.Nature Immunol. 1: 433-440, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• UniProt Accession #: P26842; B2RDZ0
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
• UniProt Protein Name: CD27 antigen
• Protein Family: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7
• UniProt Entry Name: CD27_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity

Biological Process: release of cytoplasmic sequestered NF-kappaB; response to ethanol; cell surface receptor linked signal transduction; positive regulation of JNK cascade; positive regulation of B cell differentiation; immunoglobulin mediated immune response; negative regulation of caspase activity; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome 2