anti-Mouse CD27 Monoclonal Antibody | anti-CD27 antibody

Anti-Mouse CD27 PE (Clone LG.3A10) (Armenian Hamster IgG1)

Cat. Num. AAA520088

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Reactivity: Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD27; Monoclonal Antibody; Anti-Mouse CD27 PE (Clone LG.3A10) (Armenian Hamster IgG1); CD27 PE (Clone LG.3A10) (Armenian Hamster IgG1); Anti-Mouse CD27 Monoclonal Antibody; anti-CD27 antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian hamster IgG1
• Clone Number: LG.3810
• Specificity: CD27 (Mouse CD27 Receptor)
• Form/Format: PE
• Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 260

• Applicable Applications for anti-CD27 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS) (stimulating)
• Immunogen: MCD27 tag expressing clones AR0524 and AR0530
• Donor: Armenian hamster spleen
• Fusion Partner: SP2/0 Murine Myeloma Partner
• Presentation: Biotin/FITC/PE conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
• Preparation and Storage: For all formats, store at 4 degree C. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-CD27 antibody

anti-mouse CD27 Monoclonal Antibody is specific for the mouse CD27 receptor. CD27 is part of the Tumor Necrosis Factor (TNF) family of receptors and is lymphocyte specific. Murine CD27 is expressed on the great majority of both alphabeta and gammadelta T lymphocytes, on a small population of peripheral B cells and on a very small subset of B220+ cells in the bone marrow. Murine CD27 is found on all thymocytes except a subset of CD4-CD8- precursors. There are two known basic functions of CD27 signaling. First, the binding of CD27 to CD70, a protein which is also expressed in T and B cells, provides costimulatory signals in lymphocyte proliferation and immunoglobulin production. Second, the association of CD27 with intracellular Siva, a proapoptotic protein, results in the induction of apoptotic events. This clone (LG.3A10) has been reported to work in Immunohistochemistry on frozen tissue sections, Flow Cytometry and in functional studies (stimulating).

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• UniProt Accession #: P26842; B2RDZ0
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
• UniProt Protein Name: CD27 antigen
• Protein Family: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7
• UniProt Entry Name: CD27_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Subunit structure: Homodimer. Interacts with SIVA1 and TRAF2. Ref.8 Ref.9

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Found in most T-lymphocytes.

Post-translational modification: Phosphorylated.O-glycosylated with core 1 or possibly core 8 glycans. Ref.10

Involvement in disease: Lymphoproliferative syndrome 2 (LPFS2) [MIM:615122]: An autosomal recessive immunodeficiency disorder associated with persistent symptomatic EBV viremia, hypogammaglobulinemia, and impaired T cell-dependent B-cell responses and T-cell dysfunction. The phenotype is highly variable, ranging from asymptomatic borderline-low hypogammaglobulinemia, to a full-blown symptomatic systemic inflammatory response with life-threatening EBV-related complications, including hemophagocytic lymphohistiocytosis, a lymphoproliferative disorder, and malignant lymphoma requiring stem cell transplantation.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11 Ref.12

Sequence similarities: Contains 3 TNFR-Cys repeats.