CD27 recombinant protein

CD27 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8119953

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD27; CD27 Protein; Human; Recombinant (ECD; hFc Tag); Human CD27 Protein (Fc Tag); S152 Protein; S152. LPFS2 Protein; S152.LPFS2 Protein; T14 Protein; TNFRSF7 Protein; Tp55 Protein; CD27 molecule; CD27 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile192

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ala 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CD27 (NP_001233.1)(Met1-Ile192) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CD27 recombinant protein

Background: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Product Categories/Family for CD27 recombinant protein

Cancer Drug Targets

References

Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• UniProt Accession #: P26842; B2RDZ0
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
• UniProt Protein Name: CD27 antigen
• Protein Family: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7
• UniProt Entry Name: CD27_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Membrane protein, integral; Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity

Biological Process: release of cytoplasmic sequestered NF-kappaB; response to ethanol; cell surface receptor linked signal transduction; positive regulation of JNK cascade; positive regulation of B cell differentiation; negative regulation of caspase activity; immunoglobulin mediated immune response; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome 2