Immunofluorescence (IF)
(Immunofluorescence staining of Mouse CD27 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)
Rabbit anti-Mouse CD27 Monoclonal Antibody | anti-CD27 antibody
Recombinant Anti-CD27 Antibody, Rabbit Monoclonal
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
CD27; Monoclonal Antibody; Recombinant Anti-CD27 Antibody; Rabbit Monoclonal; CD27 Antibody; Rabbit MAb; CD27 molecule; Anti-S152 Antibody; Anti-Tnfrsf7 Antibody; Anti-Tp55 Antibody; anti-CD27 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
012
Specificity
Mouse CD27
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD27 antibody
Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse CD27 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD27 (rM CD27).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of Mouse CD27 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Mouse CD27 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.)
Flow Cytometry (FC/FACS)
(Expression of CD27 on mouse splenocytes. BABL/c splenocytes were stained with purified mAb R012, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD FACSCalibur' flow cytometry system.)
Flow Cytometry (FC/FACS)
(Expression of CD27 on mouse splenocytes. BABL/c splenocytes were stained with purified mAb R012, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD FACSCalibur' flow cytometry system.)
Related Product Information for anti-CD27 antibody
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Product Categories/Family for anti-CD27 antibody
References
Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,164 Da
NCBI Official Full Name
CD27 antigen isoform a
NCBI Official Synonym Full Names
CD27 antigen
NCBI Official Symbol
Cd27
NCBI Official Synonym Symbols
S152; Tp55; Tnfrsf7
NCBI Protein Information
CD27 antigen
UniProt Protein Name
CD27 antigen
Protein Family
UniProt Gene Name
Cd27
UniProt Synonym Gene Names
Tnfrsf7
UniProt Entry Name
CD27_MOUSE
Uniprot Description
CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis
Cellular Component: cell surface; membrane; cell; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; caspase inhibitor activity
Biological Process: B cell proliferation; positive regulation of T cell differentiation; apoptosis; immunoglobulin secretion; positive regulation of JNK cascade; negative regulation of caspase activity; negative regulation of apoptosis; positive regulation of interferon-gamma biosynthetic process
Research Articles on CD27
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Product Notes
The CD27 cd27 (Catalog #AAA8110995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD27 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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