CD27 active protein

CD27 Protein, Mouse, Recombinant (His & hFc Tag)

Cat. Num. AAA8122534

• Gene Names: Cd27; S152; Tp55; Tnfrsf7
• Purity: >94% as determined by SDS-PAGE
• Synonyms: CD27; CD27 Protein; Mouse; Recombinant (His & hFc Tag); Mouse CD27/TNFRSF7 Protein (His & Fc Tag); S152 Protein; Tnfrsf7 Protein; Tp55 Protein; CD27 molecule; CD27 active protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg182

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2ug/ml (100uL/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 21
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CD27 active protein

Background: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Product Categories/Family for CD27 active protein

Cancer Drug Targets

References

Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.

NCBI and Uniprot Product Information

• NCBI GI #: 75677534
• NCBI GeneID: 21940
• NCBI Accession #: NP_001028298.1
• NCBI GenBank Nucleotide #: NM_001033126.2
• UniProt Accession #: P41272
• Molecular Weight: 28,164 Da
• NCBI Official Full Name: CD27 antigen isoform a
• NCBI Official Synonym Full Names: CD27 antigen
• NCBI Official Symbol: Cd27
• NCBI Official Synonym Symbols: S152; Tp55; Tnfrsf7
• NCBI Protein Information: CD27 antigen
• UniProt Protein Name: CD27 antigen
• Protein Family: CD27 antigen
• UniProt Gene Name: Cd27
• UniProt Synonym Gene Names: Tnfrsf7
• UniProt Entry Name: CD27_MOUSE

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis

Cellular Component: cell surface; membrane; cell; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; caspase inhibitor activity

Biological Process: B cell proliferation; positive regulation of T cell differentiation; apoptosis; immunoglobulin secretion; positive regulation of JNK cascade; negative regulation of caspase activity; negative regulation of apoptosis; positive regulation of interferon-gamma biosynthetic process