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SDS-PAGE

CD27 / TNFRSF7 recombinant protein

Recombinant Mouse CD27 / TNFRSF7 Protein (His & Fc tag)

Gene Names
Cd27; S152; Tp55; Tnfrsf7
Purity
> 94 % as determined by SDS-PAGE
Synonyms
CD27 / TNFRSF7; Recombinant Mouse CD27 / TNFRSF7 Protein (His & Fc tag); S152; Tnfrsf7; Tp55; CD27 / TNFRSF7 recombinant protein
Ordering
For Research Use Only!
Host
Human Cells
Purity/Purification
> 94 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
250
Application Notes
The recombinant mouse CD27/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 410 amino acids and has a predicted molecular mass of 46.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD27/Fc monomer is approximately 60-65 kDa due to glycosylation.
Predicted N Terminal
Thr 21
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for CD27 / TNFRSF7 recombinant protein
Background: CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Description: A DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,164 Da
NCBI Official Full Name
CD27 antigen isoform a
NCBI Official Synonym Full Names
CD27 antigen
NCBI Official Symbol
Cd27
NCBI Official Synonym Symbols
S152; Tp55; Tnfrsf7
NCBI Protein Information
CD27 antigen
UniProt Protein Name
CD27 antigen
UniProt Gene Name
Cd27
UniProt Synonym Gene Names
Tnfrsf7

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Apoptosis; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 6 F2|6 59.32 cM

Cellular Component: cell surface; external side of plasma membrane; integral to plasma membrane; membrane; neuron projection; nucleus

Molecular Function: caspase inhibitor activity; protein binding; tumor necrosis factor receptor activity

Biological Process: brain development; immune response; induction of apoptosis via death domain receptors; inflammatory response; negative regulation of apoptosis; positive regulation of JNK cascade; regulation of caspase activity; regulation of cell proliferation; response to lipopolysaccharide

Research Articles on CD27 / TNFRSF7

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Product Notes

The CD27 / TNFRSF7 cd27 (Catalog #AAA2547157) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse CD27/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 410 amino acids and has a predicted molecular mass of 46.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD27/Fc monomer is approximately 60-65 kDa due to glycosylation. Researchers should empirically determine the suitability of the CD27 / TNFRSF7 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27 / TNFRSF7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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