Mouse CD26 Monoclonal Antibody | anti-DPP4 antibody

CD26 (DPP IV / ADA-Binding Protein) Mouse Monoclonal Antibody

Cat. Num. AAA439085

• Gene Names: DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
• Reactivity: Human.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: CD26; Monoclonal Antibody; CD26 (DPP IV / ADA-Binding Protein) Mouse Monoclonal Antibody; ADA-binding protein (ADABP); Adenosine deaminase complexing protein 2 (ADCP-2); Dipeptidyl peptidase 4; Dipeptidyl peptidase 4 soluble form; Dipeptidyl peptidase IV membrane form; Dipeptidyl peptidase IV soluble form; Dipeptidyl peptidase; intestinal; Dipeptidylpeptidase 4; Dipeptidylpeptidase IV; DPP4; DPPIV; Intestinal dipeptidyl peptidase; T-cell activation antigen CD26; TP103; anti-DPP4 antibody

• Host: Mouse
• Reactivity: Human.; Others not tested
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: 134-2C2
• Specificity: Recognizes a glycoprotein of 110kDa, identified as CD26. It is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 766

• Applicable Applications for anti-DPP4 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Frozen Only) (0.5-1.0ug/ml for 30 minutes at RT)
• Cellular Localization: Cell Surface
• Immunogen: A synthetic peptide from the amino terminal region of CD26
• Hu-Chromosome Location: 2q24.3
• Positive Control: U-87 MG (Human glioblastoma astrocytoma), Ramos (Human Burkitt's lymphoma, HEP-G2 cells, and lymphocytes. Lymph nodes and tonsils.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Fleischer B. CD26: a surface protease involved in T-cell activation. Immunol Today 1994, 15(4):180-184. Abbott CA. et. al. Genomic organization, exact localization, and tissue expression of the human CD26 (dipeptidyl peptidase IV) gene. Immunogenetics 1994, 40:331-338. Valenzuela A et. al. HIV-1 envelope gp120 and viral particles block adenosine deaminase binding to human CD26. Adv Exp Med Biol 1997, 421:185-192. Cheng HC, et al. Lung endothelial dipeptidyl peptidase IV promotes adhesion and metastasis of rat breast cancer cells via tumor cell surface-associated fibronectin. J Biol Chem. 1998 273(37):24207-24015

NCBI and Uniprot Product Information

• NCBI GI #: 18765694
• NCBI GeneID: 1803
• NCBI Accession #: NP_001926.2
• NCBI GenBank Nucleotide #: NM_001935.3
• UniProt Accession #: P27487; Q53TN1
• Molecular Weight: 110kDa
• NCBI Official Full Name: dipeptidyl peptidase 4
• NCBI Official Synonym Full Names: dipeptidyl peptidase 4
• NCBI Official Symbol: DPP4
• NCBI Official Synonym Symbols: CD26; ADABP; ADCP2; DPPIV; TP103
• NCBI Protein Information: dipeptidyl peptidase 4
• UniProt Protein Name: Dipeptidyl peptidase 4
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: DPP4
• UniProt Synonym Gene Names: ADCP2; CD26; ADCP-2; DPP IV
• UniProt Entry Name: DPP4_HUMAN

NCBI Description

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

Uniprot Description

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.

Protein type: Cell adhesion; EC 3.4.14.5; Membrane protein, integral; Cell surface; Protease

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: apical plasma membrane; cell surface; endocytic vesicle; focal adhesion; lamellipodium; lysosomal membrane; membrane; plasma membrane

Molecular Function: dipeptidyl-peptidase activity; identical protein binding; protease binding; protein binding; protein homodimerization activity; receptor binding; serine-type peptidase activity; viral receptor activity

Biological Process: endothelial cell migration; positive regulation of cell proliferation; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation; T cell costimulation