Rat Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit | NPY5R elisa kit

Rat Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit

Cat. Num. AAA2705986

• Gene Names: NPY5R; NPYR5; NPY5-R; NPYY5-R
• Reactivity: Rat
• Synonyms: Neuropeptide Y Receptor Y5 (NPY5R); Rat Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit; NPYR5; NPY-Y5 receptor; NPY5R elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y Receptor Y5 (NPY5R).; No significant cross-reactivity or interference between Neuropeptide Y Receptor Y5 (NPY5R) and analogues was observed.
• Sequence Length: 445

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.051ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y Receptor Y5 (NPY5R) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y Receptor Y5 (NPY5R) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPY5R elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y Receptor Y5 (NPY5R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropeptide Y Receptor Y5 (NPY5R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide Y Receptor Y5 (NPY5R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide Y Receptor Y5 (NPY5R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32481997
• NCBI GeneID: 4889
• UniProt Accession #: Q15761; Q6GTR7; Q92916
• Molecular Weight: 50,727 Da
• NCBI Official Full Name: neuropeptide Y receptor Y5
• NCBI Official Synonym Full Names: neuropeptide Y receptor Y5
• NCBI Official Symbol: NPY5R
• NCBI Official Synonym Symbols: NPYR5; NPY5-R; NPYY5-R
• NCBI Protein Information: neuropeptide Y receptor type 5
• UniProt Protein Name: Neuropeptide Y receptor type 5
• Protein Family: Neuropeptide Y receptor
• UniProt Gene Name: NPY5R
• UniProt Synonym Gene Names: NPYR5; NPY5-R; NPYY5-R; Y5 receptor

NCBI Description

The protein encoded by this gene is a receptor for neuropeptide Y and peptide YY. The encoded protein appears to be involved in regulating food intake, with defects in this gene being associated with eating disorders. Also, the encoded protein is involved in a pathway that protects neuroblastoma cells from chemotherapy-induced cell death, providing a possible therapeutic target against neuroblastoma. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.