Testing Data
CD252 protein
CD252(OX40L)-muCD8-Biotin
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Synonyms
CD252; CD252(OX40L)-muCD8-Biotin; Human CD252(CD134L)-muCD8/Biotin; CD252 protein
Host
CHO cells
Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
0.5 mg/ml (varies by lot)
Applicable Applications for CD252 protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Molecular Structure
A soluble molecule consisting of the extracellular domain (132 aa) of human CD252 fused to the extracellular domain (167aa) of murine CD8 alpha.
Transfectant Cell Line
CHO
Performance
Five x 105 cultured human HPB-MLT cells per tube were washed and incubated 45 minutes on ice with 80 ul of CD252-muCD8/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a buffer control. Binding was blocked when reagent was pre incubated with anti-CD252 antibody at a concentration of 100 ug/ml.
Conjugation
Biotin
Production
Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Testing Data
Testing Data
Related Product Information for CD252 protein
Information: Human CD252 (CD134L, OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 is involved with costimulatory activity between B cells and T cells (1). CD252-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.
References
1. W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2. L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3. A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.
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Product Notes
The CD252 (Catalog #AAA666846) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The CD252(OX40L)-muCD8-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD252 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD252 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD252, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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