Rabbit anti-Mouse CD25/IL2RA Monoclonal Antibody | anti-IL2RA antibody

Recombinant Anti-CD25/IL2RA Antibody (APC), Rabbit Monoclonal

Cat. Num. AAA8111330

• Gene Names: Il2ra; CD25; Il2r; Ly-43
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD25/IL2RA; Monoclonal Antibody; Recombinant Anti-CD25/IL2RA Antibody (APC); Rabbit Monoclonal; CD25/IL2RA Antibody (APC); Rabbit MAb; interleukin 2 receptor; alpha; Anti-CD25 Antibody; Anti-Il2r Antibody; Anti-Ly-43 Antibody; anti-IL2RA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 049
• Specificity: Mouse CD25/IL2RA
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 2ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-IL2RA antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse CD25/IL2RA protein
• Conjugation: APC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD25/IL2RA (rM CD25/IL2RA) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of mouse IL2RA(CD25) expression on mouse splenocytes. BABL/c splenocytes were stained with APC-conjugated anti-Mouse IL2RA(CD25) and FITC-conjugated anti-Mouse CD4(BD Pharmingen).)

Related Product Information for anti-IL2RA antibody

CD25 (alpha-chain of the IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high-affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates the intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a piece of strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development are associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of SIL-2Ralpha. In most hematological malignancies, including different types of leukemias and lymphomas, SIL-2Ralpha is released directly from the surface of neoplastic cells thus reflecting the tumor bulk, turnover, and activity. Several studies have proved that not only lymphoid cancer cells but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, esophagus, and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and many solid tumors.

Product Categories/Family for anti-IL2RA antibody

Neuroinflammation Cancer Drug Targets; AKT Signaling Pathway

References

Driesen J, et al. (2008) CD25 as an immune regulatory molecule expressed on myeloid dendritic cells. Immunobiology. 213(9-10): 849-58.
Olejniczak K, et al. (2008) Biological properties of interleukin 2 and its role in pathogenesis of selected diseases--a review. Med Sci Monit. 14(10): RA179-89.
Chistiakov DA, et al. (2008) The crucial role of IL-2/IL-2RA-mediated immune regulation in the pathogenesis of type 1 diabetes, an evidence coming from genetic and animal model studies. Immunol Lett. 118(1): 1-5.
Bien E, et al. (2008) Serum soluble interleukin 2 receptor alpha in human cancer of adults and children: a review. Biomarkers. 13(1): 1-26.

NCBI and Uniprot Product Information

• NCBI GI #: 254540187
• NCBI GeneID: 16184
• NCBI Accession #: NP_032393.3
• NCBI GenBank Nucleotide #: NM_008367.3
• UniProt Accession #: P01590; Q61731
• Molecular Weight: 30,723 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha chain
• NCBI Official Symbol: Il2ra
• NCBI Official Synonym Symbols: CD25; Il2r; Ly-43
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2-RA; p55 chain
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• Protein Family: Interleukin-2 receptor
• UniProt Gene Name: Il2ra
• UniProt Synonym Gene Names: Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_MOUSE

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; positive regulation of T cell differentiation; negative regulation of inflammatory response; T cell homeostasis; positive regulation of T cell proliferation; immune response; activated T cell apoptosis