IL2RA / CD25 recombinant protein

Recombinant Mouse IL2RA / CD25 Protein (His tag)

Cat. Num. AAA2547052

• Gene Names: Il2ra; CD25; Il2r; Ly-43
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: IL2RA / CD25; Recombinant Mouse IL2RA / CD25 Protein (His tag); CD25; Il2r; Ly-43; MGC130561; IL2RA / CD25 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 268

• Application Notes: The secreted recombinant mouse IL2RA cmprises 226 amino acids with a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of the rm IL2RA is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL2RA / CD25 recombinant protein

Background: CD25 (alpha-chain of IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development is associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of sIL-2Ralpha. In most haematological malignancies, including different type s of leukaemias and lymphomas, sIL-2Ralpha has been found to be released directly from the surface of neoplastic cells thus reflecting the tumour bulk, turnover and activity. Several studies have proved that not only lymphoid cancer cells, but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, oesophagus and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and in many solid tumors.

Description: A DNA sequence encoding the mouse IL2RA (NP_032393.3) extracellular domain (Met 1-Lys 236) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 254540187
• NCBI GeneID: 16184
• NCBI Accession #: NP_032393.3
• NCBI GenBank Nucleotide #: NM_008367.3
• UniProt Accession #: P01590; Q61731
• Molecular Weight: 30,723 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha chain
• NCBI Official Symbol: Il2ra
• NCBI Official Synonym Symbols: CD25; Il2r; Ly-43
• NCBI Protein Information: interleukin-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: Il2ra
• UniProt Synonym Gene Names: Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2 A1|2 8.91 cM

Cellular Component: cell surface; external side of plasma membrane

Molecular Function: drug binding; interleukin-2 binding; interleukin-2 receptor activity; protein binding

Biological Process: activated T cell apoptosis; immune response; inflammatory response to antigenic stimulus; negative regulation of defense response to virus; negative regulation of immune response; negative regulation of inflammatory response; negative regulation of lymphocyte proliferation; negative regulation of T cell proliferation; Notch signaling pathway; positive regulation of activated T cell proliferation; positive regulation of T cell differentiation; positive regulation of T cell proliferation; regulation of T cell homeostatic proliferation; regulation of T cell tolerance induction; T cell homeostasis