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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD226 : Low Endotoxin (MBS211218))

Mouse CD226 Monoclonal Antibody | anti-CD226 antibody

MOUSE ANTI HUMAN CD226:Biotin

Gene Names
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD226; Monoclonal Antibody; MOUSE ANTI HUMAN CD226:Biotin; anti-CD226 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DX11
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
336
Applicable Applications for anti-CD226 antibody
Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Perservative Stabilisers
0.09% Sodium Azide
1.0% Bovine Serum Albumin
Preparation
Immunogen
Human cytotoxic T lymphocyte clone
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD226 : Low Endotoxin (MBS211218))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD226 : Low Endotoxin (MBS211218))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:RPE (MBS213765))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:RPE (MBS213765))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:FITC (MBS210904))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:FITC (MBS210904))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:Azide Free (MBS210234))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD226:Azide Free (MBS210234))
Related Product Information for anti-CD226 antibody
Mouse anti Human CD226 antibody, clone DX11 recognizes human CD226, a 65kDa glycoprotein, also known as DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes. Mouse anti Human CD226 antibody, clone DX11 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,614 Da
NCBI Official Full Name
CD226 antigen isoform a
NCBI Official Synonym Full Names
CD226 molecule
NCBI Official Symbol
CD226
NCBI Official Synonym Symbols
PTA1; DNAM1; DNAM-1; TLiSA1
NCBI Protein Information
CD226 antigen; DNAX accessory molecule 1; DNAX accessory molecule-1; T lineage-specific activation antigen 1 antigen; adhesion glycoprotein; platelet and T cell activation antigen 1
UniProt Protein Name
CD226 antigen
Protein Family
UniProt Gene Name
CD226
UniProt Synonym Gene Names
DNAM1; DNAM-1
UniProt Entry Name
CD226_HUMAN

NCBI Description

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

DNAM-1: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q22.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; protein binding; cell adhesion molecule binding; protein kinase binding

Biological Process: regulation of immune response; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of immunoglobulin mediated immune response; cytokine production; cell recognition; positive regulation of natural killer cell mediated cytotoxicity; signal transduction; cell adhesion; positive regulation of mast cell activation

Research Articles on CD226

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Product Notes

The CD226 cd226 (Catalog #AAA210428) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD226 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD226 cd226 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD226, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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