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Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212645))

Rat CD200R Monoclonal Antibody | anti-CD200R antibody

RAT ANTI MOUSE CD200R:FITC

Gene Names
Cd200r1; OX2R; Mox2r; CD200R
Applications
Flow Cytometry, Functional Assay
Synonyms
CD200R; Monoclonal Antibody; RAT ANTI MOUSE CD200R:FITC; anti-CD200R antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
OX-110
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Sequence Length
326
Applicable Applications for anti-CD200R antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212645))

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212645))

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R: FITC)

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R: FITC)

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212646))

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R (MBS212646))

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R: Alexa Fluor 488 (MBS212645A488))

Testing Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD200R: Alexa Fluor 488 (MBS212645A488))

Testing Data

(Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD200R: RPE (MBS213768))

Testing Data (Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD200R: RPE (MBS213768))

Testing Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD200R: Low Endotoxin (MBS211221))

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD200R: Low Endotoxin (MBS211221))
Related Product Information for anti-CD200R antibody
Rat anti Mouse CD200R antibody, clone OX-110 recognizes mouse CD200R, a cell surface glycoprotein (also known as OX2R. Mouse CD200R is a 326 amino acid, ~48kDa single pass type I transmembrane glycoprotein, expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells (Wright et al.2003). Studies suggest that CD200-CD200R interaction may be involved in the control of myeloid cellular function (Minas and Liversidge 2006). Rat anti Mouse CD200R antibody, clone OX-110 has been used successfully for the immunohistochemical detection of CD200R expressing cells on acetone fixed cryosections of murine synovial tissue (Simelyte et al. 2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,505 Da
NCBI Official Full Name
cell surface glycoprotein CD200 receptor 1
NCBI Official Synonym Full Names
CD200 receptor 1
NCBI Official Symbol
Cd200r1
NCBI Official Synonym Symbols
OX2R; Mox2r; CD200R
NCBI Protein Information
cell surface glycoprotein CD200 receptor 1; CD200 cell surface glycoprotein receptor; Orexin receptor 2; antigen identified by monoclonal antibody MRC OX-2 receptor; cell surface glycoprotein OX2 receptor 1
UniProt Protein Name
Cell surface glycoprotein CD200 receptor 1
UniProt Gene Name
Cd200r1
UniProt Synonym Gene Names
Mox2r; Ox2r
UniProt Entry Name
MO2R1_MOUSE

Uniprot Description

CD200R: Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. Belongs to the CD200R family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: membrane; plasma membrane; integral to membrane; receptor complex; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Research Articles on CD200R

Similar Products

Product Notes

The CD200R cd200r1 (Catalog #AAA210911) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD200R can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20. Researchers should empirically determine the suitability of the CD200R cd200r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD200R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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