ELISA (EIA)
(Detection of human myoglobin in sandwich ELISA using Mouse anti Human myoglobin as the capture reagent and HRP-conjugated Mouse anti Human myoglobin as the detection reagent. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.)
Mouse anti-Human MYOGLOBIN Monoclonal Antibody
MOUSE ANTI HUMAN MYOGLOBIN
Reactivity
Human
Applications
ELISA
Synonyms
MYOGLOBIN; Monoclonal Antibody; MOUSE ANTI HUMAN MYOGLOBIN; Myoglobin antibody|164.3D5; anti-MYOGLOBIN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
164.3D5
Specificity
MYOGLOBIN
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-MYOGLOBIN antibody
ELISA (EIA)
Application Notes
ELISA: Max Dilution: 5ug/ml
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
ELISA (EIA)
(Detection of human myoglobin in sandwich ELISA using Mouse anti Human myoglobin as the capture reagent and HRP-conjugated Mouse anti Human myoglobin as the detection reagent. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.)
ELISA (EIA)
(Detection of human myoglobin in sandwich ELISA using Mouse anti Human myoglobin as the capture reagent and HRP-conjugated Mouse anti Human myoglobin as the detection reagent. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.)
Related Product Information for anti-MYOGLOBIN antibody
Mouse anti Human myoglobin, clone 164.3D5 is a monoclonal antibody recognizing human myoglobin, a single-chain globular ~17 kDa protein containing a heme prosthetic group localized to skeletal muscle. Myoglobin is responsible for the storage and movement of oxygen in muscle tissue.
Myoglobin is released from injured muscle into the bloodstream, making it a sensitive marker for all muscle injury. It is commonly used in combination with troponin as a marker for myocardial damage in heart attacks. It is filtered by the kidneys, but is a potential cause of renal failure as it is toxic to the renal tubular epithelium (Lima et al. 2008).
Myoglobin is released from injured muscle into the bloodstream, making it a sensitive marker for all muscle injury. It is commonly used in combination with troponin as a marker for myocardial damage in heart attacks. It is filtered by the kidneys, but is a potential cause of renal failure as it is toxic to the renal tubular epithelium (Lima et al. 2008).
Similar Products
Product Notes
The MYOGLOBIN (Catalog #AAA226007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN MYOGLOBIN reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYOGLOBIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: Max Dilution: 5ug/ml Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the MYOGLOBIN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYOGLOBIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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