Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Testing Data #1 (Immunoperoxidase staining of LEW rat spleen follicle with Mouse anti Rat CD1d (MBS224737))

Mouse CD1d Monoclonal Antibody | anti-CD1d antibody

MOUSE ANTI RAT CD1d

Gene Names
Cd1d1; Cd1a; Cd1d; CD1.1; Ly-38; AI747460
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
CD1d; Monoclonal Antibody; MOUSE ANTI RAT CD1d; anti-CD1d antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
WTH1
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (varies by lot)
Sequence Length
336
Applicable Applications for anti-CD1d antibody
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/2,000
Western Blotting: Application Note: Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details.
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation
Immunogen
Rat CD1d-transduced M12.4.1.C3 cells.
Buffer Solution
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data #1

(Immunoperoxidase staining of LEW rat spleen follicle with Mouse anti Rat CD1d (MBS224737))

Testing Data #1 (Immunoperoxidase staining of LEW rat spleen follicle with Mouse anti Rat CD1d (MBS224737))

Testing Data #2

(Staining of rat thymocytes with Mouse anti Rat CD1d:FITC (MBS224645))

Testing Data #2 (Staining of rat thymocytes with Mouse anti Rat CD1d:FITC (MBS224645))
Related Product Information for anti-CD1d antibody
Mouse anti Rat CD1d antibody, clone WTH1 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines. Clone WTH1 recognizes a different, non-overlapping epitope to clone WTH2 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2. Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,554 Da
NCBI Official Full Name
antigen-presenting glycoprotein CD1d1
NCBI Official Synonym Full Names
CD1d1 antigen
NCBI Official Symbol
Cd1d1
NCBI Official Synonym Symbols
Cd1a; Cd1d; CD1.1; Ly-38; AI747460
NCBI Protein Information
antigen-presenting glycoprotein CD1d1; T-cell surface glycoprotein CD1d1
UniProt Protein Name
Antigen-presenting glycoprotein CD1d1
UniProt Gene Name
Cd1d1
UniProt Synonym Gene Names
Cd1.1
UniProt Entry Name
CD1D1_MOUSE

Uniprot Description

CD1D: Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

Protein type: Cell surface; Apoptosis; Lipid-binding; Membrane protein, integral

Cellular Component: cell surface; membrane; lysosome; early endosome; late endosome; cytoplasm; plasma membrane; integral to membrane; endosome; external side of plasma membrane

Molecular Function: protein binding; histone binding; beta-2-microglobulin binding; exogenous lipid antigen binding; T cell receptor binding; lipid antigen binding; cell adhesion molecule binding; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, endogenous lipid antigen via MHC class Ib; positive regulation of NK T cell activation; regulation of immune response; positive regulation of T cell mediated cytotoxicity; immune system process; positive regulation of interleukin-2 biosynthetic process; antigen processing and presentation; positive regulation of interleukin-4 biosynthetic process; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; innate immune response; positive regulation of T cell proliferation; immune response; positive regulation of NK T cell differentiation; positive regulation of interferon-gamma biosynthetic process; positive thymic T cell selection; positive regulation of macrophage activation

Research Articles on CD1d

Similar Products

Product Notes

The CD1d cd1d1 (Catalog #AAA224453) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD1d can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/2,000 Western Blotting: Application Note: Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details. Researchers should empirically determine the suitability of the CD1d cd1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD1d, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.