Rabbit Nephrin ELISA Kit | NPHS1 elisa kit

Rabbit Nephrin ELISA Kit

Cat. Num. AAA726374

• Gene Names: NPHS1; CNF; NPHN; nephrin
• Reactivity: Rabbit
• Synonyms: Nephrin; Rabbit Nephrin ELISA Kit; NPHS1 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This NEPHRIN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit NEPHRIN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPHS1 elisa kit

Principle of the Assay: NEPHRIN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NEPHRIN antibody and an NEPHRIN-HRP conjugate. The assay sample and buffer are incubated together with NEPHRIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEPHRIN concentration since NEPHRIN from samples and NEPHRIN-HRP conjugate compete for the anti-NEPHRIN antibody binding site. Since the number of sites is limited, as more sites are occupied by NEPHRIN from the sample, fewer sites are left to bind NEPHRIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEPHRIN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NPHS1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 10441644
• NCBI GeneID: 4868
• UniProt Accession #: O60500; A6NDH2; C3RX61
• Molecular Weight: 134,742 Da
• NCBI Official Full Name: nephrin
• NCBI Official Synonym Full Names: nephrosis 1, congenital, Finnish type (nephrin)
• NCBI Official Symbol: NPHS1
• NCBI Official Synonym Symbols: CNF; NPHN; nephrin
• NCBI Protein Information: nephrin; renal glomerulus-specific cell adhesion receptor
• UniProt Protein Name: Nephrin
• Protein Family: Nephrin
• UniProt Gene Name: NPHS1
• UniProt Synonym Gene Names: NPHN
• UniProt Entry Name: NPHN_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

Uniprot Description

Function: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion

By similarity.

Subunit structure: Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1

By similarity. Interacts with NPHS2. Ref.7

Subcellular location: Cell membrane; Single-pass type I membrane protein

Potential. Note: Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. Ref.5 Ref.6

Tissue specificity: Specifically expressed in podocytes of kidney glomeruli.

Developmental stage: In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.

Post-translational modification: Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1

By similarity. Ref.8

Involvement in disease: Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [

MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Ref.1 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

Sequence similarities: Belongs to the immunoglobulin superfamily.Contains 1 fibronectin type-III domain.Contains 8 Ig-like C2-type (immunoglobulin-like) domains.