Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178 (MBS212780))
Mouse anti-Human CD178 Monoclonal Antibody | anti-CD178 antibody
MOUSE ANTI HUMAN CD178
CD178 is expressed by activated T lymphocytes and NK cells (Leite-de-Moraes and Dy 1997). The protein may exist as either a membrane bound or a cleaved soluble form (Garcia et al. 2013). CD178 plays an important role in T cell mediated cytotoxicity (Jodo et al. 2005). Binding of CD178 to Fas (CD95) results in the induction of apoptosis (Ju et al. 1995).
Mouse anti human CD178 antibody, clone 14C2 is reported to recognize a conformation dependent non-blocking epitope on CD178 (Daburon et al. 2013).
- Suggested Dilution: 1/25 - 1/50
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Results maybe enhanced using membrane permeabilization. The use of Leucoperm™ (Please Inquire) prior to staining is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178 (MBS212780))
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178 (MBS212780))
Testing Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178: Alexa Fluor 647 (MBS212780A647))
Testing Data
(Staining of CD178 transfected CHO cells with Mouse anti Human CD178: Alexa Fluor 647 (MBS212780A647))
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178)
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178)
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178:Alexa Fluor 488 (MBS212780A488))
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178:Alexa Fluor 488 (MBS212780A488))
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178:RPE (MBS213807))
Testing Data
(Staining of permeabilized Jurkat cells with Mouse anti Human CD178:RPE (MBS213807))
Testing Data
(Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MBS212780) as a capture reagent and biotinylated Mouse anti Human CD178 (MBS212778) as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)
Testing Data
(Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MBS212780) as a capture reagent and biotinylated Mouse anti Human CD178 (MBS212778) as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]
Uniprot Description
FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Apoptosis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: lysosomal lumen; extracellular space; perinuclear region of cytoplasm; integral to plasma membrane; extracellular region; plasma membrane; caveola; nucleus; external side of plasma membrane
Molecular Function: protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; cell-cell signaling; induction of apoptosis via death domain receptors; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; retinal cell programmed cell death; immune response
Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome
Research Articles on CD178
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Product Notes
The CD178 faslg (Catalog #AAA212781) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD178 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD178 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: - Suggested Dilution: 1/25 - 1/50 - Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. - Results maybe enhanced using membrane permeabilization. The use of Leucoperm™ (Please Inquire) prior to staining is recommended. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD178 faslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD178, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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