Mouse Interferon Regulatory Factor4 (IRF4) ELISA Kit | IRF4 elisa kit

Mouse Interferon Regulatory Factor4 (IRF4) ELISA Kit

Cat. Num. AAA2614184

• Gene Names: IRF4; MUM1; LSIRF; SHEP8; NF-EM5
• Reactivity: Mouse
• Synonyms: Interferon Regulatory Factor4 (IRF4); Mouse Interferon Regulatory Factor4 (IRF4) ELISA Kit; IRF4 elisa kit

• Reactivity: Mouse
• Sequence Length: 450

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 2000 pg/mL-31.2 pg/mL
• Sensitivity: Up to 12 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRF4 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse IRF4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 305632828
• NCBI GeneID: 3662
• NCBI GenBank Nucleotide #: NP_001182215.1
• UniProt Accession #: Q15306
• NCBI Official Full Name: interferon regulatory factor 4 isoform 2
• NCBI Official Synonym Full Names: interferon regulatory factor 4
• NCBI Official Symbol: IRF4
• NCBI Official Synonym Symbols: MUM1; LSIRF; SHEP8; NF-EM5
• NCBI Protein Information: interferon regulatory factor 4
• UniProt Protein Name: Interferon regulatory factor 4
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF4
• UniProt Synonym Gene Names: MUM1; IRF-4; LSIRF
• UniProt Entry Name: IRF4_HUMAN

NCBI Description

The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

IRF4: Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Interacts with SPIB and DEF6. Not induced by interferons. Lymphoid cells. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 6p25-p23

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; sequence-specific DNA binding; protein-lysine N-methyltransferase activity; transcription factor binding; transcription factor activity

Biological Process: T cell activation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; regulation of T-helper cell differentiation; myeloid dendritic cell differentiation; defense response to protozoan; peptidyl-lysine methylation; positive regulation of interleukin-4 biosynthetic process; positive regulation of interleukin-10 biosynthetic process; positive regulation of interleukin-13 biosynthetic process; negative regulation of toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA binding

Disease: Skin/hair/eye Pigmentation, Variation In, 8