Mouse anti-Human CD172b Monoclonal Antibody | anti-CD172b antibody

Anti-Hu CD172b Purified

Cat. Num. AAA1800387

• Gene Names: SIRPB1; CD172b; SIRP-BETA-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD172b; Monoclonal Antibody; Anti-Hu CD172b Purified; signal regulatory protein beta 1; SIRPB1; anti-CD172b antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: B4B6
• Specificity: The mouse monoclonal antibody B4B6 recognizes an extracellular epitope of CD172b, an approximately 50 kDa transmembrane glycoprotein expressed on myeloid cells.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 181

• Applicable Applications for anti-CD172b antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: NIH-3T3 cells transfected with human CD172b
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD172b (B4B6) purified, GAM-APC.)

Related Product Information for anti-CD172b antibody

CD172b, the signal-regulatory protein beta (SIRP beta) is a disulfide-linked homodimer expressed on myeloid cells including monocytes and dendritic cells. Similarly to CD172a, it serves as a negative regulator of tyrosine kinase-coupled signaling processes. Unlike CD172a, the CD172b protein does not possess the cytoplasmic domain, but instead its transmembrane domain can interact with another transmembrane protein DAP-12, which contains ITAM sequences in its intracellular domain and links CD172b to the downstream signaling molecules. The result is e.g. regulation of neutrophil transepithelial migration.

NCBI and Uniprot Product Information

• NCBI GI #: 144953878
• NCBI GeneID: 10326
• NCBI Accession #: NP_001077379.1
• NCBI GenBank Nucleotide #: NP_001077379.1
• UniProt Accession #: O00241; Q5TFQ8
• NCBI Official Full Name: signal-regulatory protein beta-1 isoform 2
• NCBI Official Synonym Full Names: signal regulatory protein beta 1
• NCBI Official Symbol: SIRPB1
• NCBI Official Synonym Symbols: CD172b; SIRP-BETA-1
• NCBI Protein Information: signal-regulatory protein beta-1
• UniProt Protein Name: Signal-regulatory protein beta-1
• UniProt Gene Name: SIRPB1
• UniProt Synonym Gene Names: SIRP-beta-1
• UniProt Entry Name: SIRB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein was found to interact with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. This protein was also reported to participate in the recruitment of tyrosine kinase SYK. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

SIRPB1: Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; innate immune response; signal transduction