Western Blot (WB)
Rabbit FtsZ Polyclonal Antibody | anti-FtsZ antibody
FtsZ Antibody
Reactivity
Eukaryotic Proteins
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
FtsZ; Polyclonal Antibody; FtsZ Antibody; S. aureus; Ftsz; Cell division protein ftsZ; JW0093; b0095; sfiB; sulB antibody; anti-FtsZ antibody
Host
Rabbit
Reactivity
Eukaryotic Proteins
Clonality
Polyclonal
Form/Format
Affinity Purified
Sequence Length
390
Applicable Applications for anti-FtsZ antibody
ELISAP, IP, WB
Application Notes
DOT BLOT: 1: 10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500,~47-48 kDa (apparent).
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500,~47-48 kDa (apparent).
Immunogen
Synthetic peptide taken within amino acid region 360-410 on S. aureus FtsZ protein.
Immunoglobulin & Concentration
0.72mg/mL igG in antibondy stabilization buffer.
Molecular Function
GTPase activity
Structure
Homodimer. Polymerizes to form dynamic ring structure in a strictly GTP-dependent manner
Subcellular Location
Cytoplasm; Assembles midcell at the inner surface of Cytoplasmic membrane
Preparation and Storage
Store at -20°C for long term storage.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-FtsZ antibody
The bacterial cytokinetic protein FtsZ is a tubulin-like protein with GTPase activity and it plays an important role in cell division. The FtsZ protein is conserved throughout the bacteria and archaea where in response to an unknown cell division signal it polymerizes into a ring which becomes the site of future cell division site. During cell division of B. subtilis, the frequency of cells with FtsZ rings varies 2-3-fold, however, the cellular concentration of FtsZ remain constant irrespective of doubling time (1). The FtsZ is expressed via a constitutive promoter thus eliminating the normal transcription al control. The FtsZ undergo GTP-dependent polymerization into medical FtsZ ring-formation and separation at cell poles is mediated by several protein partners. The MinCD complex is found concentrated at the cell poles where it inhibits the FtsZ polymerization (2). The FtsZ protein has GTPase activity which correlated with the FtsZ is inhibited by sulA which is further purified as a fusion protein to MalE and staphylococcus protein A. The FtsZ deficient B. subtilis mutants are deficient in the initiation stages of cell division and exhibit long filament like growth when subjected to non-permissive temperature. The free energy of GTP hydrolysis appears not to be used for generation O constriction forces thus the role of GTPase activity in ring formation is still an open question (4). The Z ring is a very dynamic structure through which subunits of FtsZ cycle with a half time of about 10 seconds. FtsZ is a GRPase that is similar to the eukaryotic tubulins and is essential for cell division in prokaryotes. FtsZ is capable of polymerizing in a GTP-driven process into structures similar to those formed by tubulin. FtsZ forms a ring-shaped septum just prior to cell division. Staphylococcus aureus FtsZ is a 390 amino acid protein with a nucleotide binding site and a SulA interaction site. Disruption in the activity of FtsZ in S aureus affects its growth pattern and may be an important therapeutic target fight against staphylococcus infections.
The Anti-FtsZ-selective antibodies were generated against a synthetic peptide corresponding to a near C-terminal location on S.Aureus protein. The FtsZ-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded high affinity epitope-specific antibodies. The FtsZ antibodies label a protein of approximately 47-48kDa protein in Western Blot using Western Blot positive control for FtsZ.
The Anti-FtsZ-selective antibodies were generated against a synthetic peptide corresponding to a near C-terminal location on S.Aureus protein. The FtsZ-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded high affinity epitope-specific antibodies. The FtsZ antibodies label a protein of approximately 47-48kDa protein in Western Blot using Western Blot positive control for FtsZ.
Product Categories/Family for anti-FtsZ antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
~47-48 kDa (apparent)
NCBI Official Full Name
cell division protein
UniProt Protein Name
Cell division protein FtsZ
Protein Family
UniProt Gene Name
ftsZ
UniProt Entry Name
B5LV62_STAAU
Uniprot Description
Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.
Similar Products
Product Notes
The FtsZ ftsz (Catalog #AAA540208) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FtsZ Antibody reacts with Eukaryotic Proteins and may cross-react with other species as described in the data sheet. AAA Biotech's FtsZ can be used in a range of immunoassay formats including, but not limited to, ELISAP, IP, WB. DOT BLOT: 1: 10,000ELISA: 1:10,000
Immunoprecipitation: 1:200 Western Blot: 1:500,~47-48 kDa (apparent). Researchers should empirically determine the suitability of the FtsZ ftsz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FtsZ, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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