Rabbit Ksp-Cadherin (Kidney-Specific Cadherin)/CDH16 Polyclonal Antibody | anti-CDH16 antibody

Ksp-Cadherin (Kidney-Specific Cadherin)/CDH16

Cat. Num. AAA4380769

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ksp-Cadherin (Kidney-Specific Cadherin)/CDH16; Polyclonal Antibody; Cadherin-16 (CDH16); Kidney-specific cadherin; Ksp-cadherin antibody; anti-CDH16 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 807

• Applicable Applications for anti-CDH16 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Normal kidney or renal cell carcinoma.
• Immunogen: Recombinant human CDH16 protein
• Cellular Localization: Cell Surface with some cytoplasmic
• Hu-chromosome Location: 16q22.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Rabbit Polyclonal Antibody.)

References

1. Mazal, P.R., et al. 2005. Expression of kidney-specific cadherin distinguishes chromophobe renal cell carcinoma from renal oncocytoma. Hum Pathol. 36: 22-28. 2. Shen, S.S., et al. 2005. Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms. Mod Pathol. 18: 933-40.

NCBI and Uniprot Product Information

• NCBI GI #: 325296969
• NCBI GeneID: 1014
• NCBI Accession #: NP_001191673.1
• NCBI GenBank Nucleotide #: NP_001191673.1
• UniProt Accession #: O75309
• Molecular Weight: 130kDa
• NCBI Official Full Name: cadherin-16 isoform 2
• NCBI Official Synonym Full Names: cadherin 16
• NCBI Official Symbol: CDH16
• NCBI Protein Information: cadherin-16
• UniProt Protein Name: Cadherin-16
• Protein Family: Cadherin
• UniProt Gene Name: CDH16
• UniProt Synonym Gene Names: Ksp-cadherin
• UniProt Entry Name: CAD16_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

CDH16: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: basolateral plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: calcium-dependent cell-cell adhesion; cell adhesion; homophilic cell adhesion