Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Cynomolgus CD166 expression on Cynomolgus monocytes. Cells were stained with FITC-conjugated anti-CD166. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.)
Rabbit anti-Cynomolgus CD166/ALCAM Monoclonal Antibody | anti-ALCAM antibody
Recombinant Anti-CD166/ALCAM Antibody (FITC), Rabbit Monoclonal
Reactivity
Cynomolgus
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD166/ALCAM; Monoclonal Antibody; Recombinant Anti-CD166/ALCAM Antibody (FITC); Rabbit Monoclonal; CD166/ALCAM Antibody (FITC); Rabbit MAb; activated leukocyte cell adhesion molecule; anti-ALCAM antibody
Host
Rabbit
Reactivity
Cynomolgus
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
018
Specificity
Cynomolgus CD166/ALCAM
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-ALCAM antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Cynomolgus CD166/ALCAM Protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus CD166/ALCAM and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Cynomolgus CD166 expression on Cynomolgus monocytes. Cells were stained with FITC-conjugated anti-CD166. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Cynomolgus CD166 expression on Cynomolgus monocytes. Cells were stained with FITC-conjugated anti-CD166. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.)
Related Product Information for anti-ALCAM antibody
Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T-and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.
References
Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.Fujiwara H, et al. (2003) Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 88(7): 3437-43.Jezierska A, et al. (2006) ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12(8): BR263-73.Kahlert C, et al. (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101(3): 457-64.
NCBI and Uniprot Product Information
UniProt Accession #
Protein Family
Similar Products
Product Notes
The ALCAM (Catalog #AAA8113266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD166/ALCAM Antibody (FITC), Rabbit Monoclonal reacts with Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's CD166/ALCAM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the ALCAM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD166/ALCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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