Integrin Alpha 5 (ITGa5) Recombinant Protein | ITGa5 recombinant protein

Recombinant Integrin Alpha 5 (ITGa5)

Cat. Num. AAA2125479

• Gene Names: ITGA5; FNRA; CD49e; VLA5A
• Applications: SDS-Page, Western Blot
• Purity: Greater than 80% by SDS-PAGE
• Synonyms: Integrin Alpha 5 (ITGa5); Recombinant Integrin Alpha 5 (ITGa5); ITGa5/Integrin Alpha 5; CD49e; CD49-E; ITG-A5; FNRA; VLA5A; Fibronectin Receptor; Alpha Polypeptide; Integrin alpha-F; CD49 antigen-like family member E; ITGa5 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 80% by SDS-PAGE
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Phe42~Asn664

• Applicable Applications for ITGa5 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Tag: N-terminal His Tag
• Expression System: Prokaryotic expression
• Organism Species: Human
• Isoelectric Point: 4.4
• Subcellular Location: Membrane
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.; The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

SDS-Page

(SDS-PAGE)

Product Categories/Family for ITGa5 recombinant protein

CD & Adhesion molecule; Prokaryotic recombinant

NCBI and Uniprot Product Information

• NCBI GI #: 56237029
• NCBI GeneID: 3678
• NCBI Accession #: NP_002196.2
• NCBI GenBank Nucleotide #: NM_002205.2
• UniProt Accession #: P08648; Q96HA5
• Molecular Weight: 114,536 Da
• NCBI Official Full Name: integrin alpha-5
• NCBI Official Synonym Full Names: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• NCBI Official Symbol: ITGA5
• NCBI Official Synonym Symbols: FNRA; CD49e; VLA5A
• NCBI Protein Information: integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit
• UniProt Protein Name: Integrin alpha-5
• Protein Family: Integrin
• UniProt Gene Name: ITGA5
• UniProt Synonym Gene Names: FNRA
• UniProt Entry Name: ITA5_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: ruffle; focal adhesion; cell surface; cytoplasm; plasma membrane; synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: integrin binding; protein binding; vascular endothelial growth factor receptor 2 binding; metal ion binding; platelet-derived growth factor receptor binding; epidermal growth factor receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; viral reproduction; cell-substrate adhesion; positive regulation of vascular endothelial growth factor receptor signaling pathway; memory; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion mediated by integrin; cell-substrate junction assembly; angiogenesis; cell adhesion; blood coagulation; leukocyte migration; wound healing, spreading of epidermal cells; positive regulation of cell migration