Mouse anti-Human CD166/ALCAM Monoclonal Antibody | anti-ALCAM antibody

Anti-CD166/ALCAM Antibody (APC), Mouse Monoclonal

Cat. Num. AAA8100387

• Gene Names: ALCAM; MEMD; CD166
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD166/ALCAM; Monoclonal Antibody; Anti-CD166/ALCAM Antibody (APC); Mouse Monoclonal; CD166/ALCAM Antibody (APC); Mouse MAb; activated leukocyte cell adhesion molecule; Anti-CD166 Antibody; Anti-MEMD Antibody; anti-ALCAM antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 03
• Specificity: Human CD166/ALCAM
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-ALCAM antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human CD166/ALCAM protein
• Conjugation: APC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD166/ALCAM (rh CD166/ALCAM) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of anti-ALCAM (CD166) reactivity on Jurkat cells analyzed by flow cytometry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD166/ALCAM expression on Jurkat cells. Cells were stained with APC-conjugated anti-Human CD166/ALCAM. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-ALCAM antibody

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T-and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

References

Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.Fujiwara H, et al. (2003) Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 88(7): 3437-43.Jezierska A, et al. (2006) ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12(8): BR263-73.Kahlert C, et al. (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101(3): 457-64.

NCBI and Uniprot Product Information

• NCBI GI #: 68163411
• NCBI GeneID: 214
• NCBI Accession #: NP_001618.2
• NCBI GenBank Nucleotide #: NM_001627.3
• UniProt Accession #: Q13740; O60892; Q1HGM8; Q1HGM9; Q6PEY4; Q6ZS95; B2RNS3; B4DTU0
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: CD166 antigen isoform 1
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: ALCAM
• UniProt Synonym Gene Names: MEMD
• UniProt Entry Name: CD166_HUMAN

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion