Rabbit anti-Cynomolgus CD166/ALCAM Monoclonal Antibody | anti-ALCAM antibody

Recombinant Anti-CD166/ALCAM Antibody, Rabbit Monoclonal

Cat. Num. AAA8113264

• Reactivity: Cynomolgus
• Applications: Western Blot
• Purity: Protein A
• Synonyms: CD166/ALCAM; Monoclonal Antibody; Recombinant Anti-CD166/ALCAM Antibody; Rabbit Monoclonal; CD166/ALCAM Antibody; Rabbit MAb; activated leukocyte cell adhesion molecule; anti-ALCAM antibody

• Host: Rabbit
• Reactivity: Cynomolgus
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 008
• Specificity: Cynomolgus CD166/ALCAM
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ALCAM antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Cynomolgus CD166/ALCAM protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus CD166/ALCAM.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ALCAM rabbit monoclonal antibody at 1:500 dilutionLane A: HuT78 Whole Cell LysateLane B: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:65 kDaObserved band size:65 kDa)

Related Product Information for anti-ALCAM antibody

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T-and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

References

Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.Fujiwara H, et al. (2003) Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 88(7): 3437-43.Jezierska A, et al. (2006) ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12(8): BR263-73.Kahlert C, et al. (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101(3): 457-64.

NCBI and Uniprot Product Information

• UniProt Accession #: G7NZQ8
• Protein Family: CD166 antigen