Mouse anti-Rat CD161 Monoclonal Antibody | anti-CD161 antibody

CD161 (3F8) Monoclonal Antibody

Cat. Num. AAA3014393

• Gene Names: KLRB1; NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CD161; Monoclonal Antibody; CD161 (3F8) Monoclonal Antibody; Killer cell lectin-like receptor subfamily B member 1; C-type lectin domain family 5 member B; HNKR-P1a; NKR-P1A; Natural killer cell surface protein P1A; anti-CD161 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Specificity: CD161 (3F8) mAb detects endogenous levels of CD161 protein.
• Purity/Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 225

• Applicable Applications for anti-CD161 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids of Human CD161.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CD 161 (NKR-PIA) mAb at 1:2000 dillutionLane1:The Peripheral blood lysate of Rat (40ug) Lane2:The Liver tissue lysate of Rat (40ug))

Related Product Information for anti-CD161 antibody

Natural killer (NK) and T cells express a superfamily of proteins with structural features of C-type lectins. T cells bearing natural killer receptors (NKRs) such as CD94 and CD161 are present in psoriasis. CD161 mediates NK cell activation and functions as an activating receptor. CD161 is a prototypic marker of NK cells, although it is also found on a subset of CD8+ T cells. The expression of NK receptors on CD8+ T cells can be considered a marker of cytotoxic effector T cells that are expanded in vivo after antigenic activation leading to extensive proliferation. The transcription, mRNA accumulation, and surface expression of CD161, a molecule involved in triggering cytotoxicity, is specifically upregulated by IL-12.

NCBI and Uniprot Product Information

• NCBI GI #: 4504879
• NCBI GeneID: 3820
• NCBI Accession #: NP_002249.1
• NCBI GenBank Nucleotide #: NP_002249.1
• UniProt Accession #: Q12918
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: killer cell lectin-like receptor subfamily B member 1
• NCBI Official Synonym Full Names: killer cell lectin like receptor B1
• NCBI Official Symbol: KLRB1
• NCBI Official Synonym Symbols: NKR; CD161; CLEC5B; NKR-P1; NKRP1A; NKR-P1A; hNKR-P1A
• NCBI Protein Information: killer cell lectin-like receptor subfamily B member 1
• UniProt Protein Name: Killer cell lectin-like receptor subfamily B member 1
• UniProt Gene Name: KLRB1
• UniProt Synonym Gene Names: CLEC5B; NKRP1A; NKR-P1A
• UniProt Entry Name: KLRB1_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

KLRB1: Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: plasma membrane; integral to membrane

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction