Goat ETFB Polyclonal Antibody | anti-ETFB antibody

Anti-ETFB Antibody (aa231-243)

Cat. Num. AAA2400847

• Gene Names: ETFB; MADD; FP585
• Reactivity: Human; Predicted: Rabbit, Mouse, Bovine, Bat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ETFB; Polyclonal Antibody; Anti-ETFB Antibody (aa231-243); Goat Polyclonal to Human ETFB; FP585; Beta-ETF; anti-ETFB antibody

• Host: Goat
• Reactivity: Human; Predicted: Rabbit, Mouse, Bovine, Bat
• Clonality: Polyclonal
• Specificity: Human ETFB. This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 255

• Applicable Applications for anti-ETFB antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:8000; IHC-P: 3.75 ug/ml; WB: 0.01-0.03 ug/ml
• Immunogen: Synthetic peptide C-PQRTAGVKVETTE from the C-terminus of human ETFB (NP_001976.1; NP_001014763.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Bat, Bovine, Rabbit (100%); Marmoset, Mouse, Rat, Hamster, Panda, Horse, Pig (92%); Elephant, Dog, Xenopus (85%).
• Conjugation: Unconjugated
• Epitope: aa231-243
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(ETFB antibody (0.01 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ETFB antibody

The electron transfer flavoprotein serves as a specific electronacceptor for several dehydrogenases, including five acyl-CoAdehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfersthe electrons to the main mitochondrial respiratory chain viaETF-ubiquinone oxidoreductase (ETF dehydrogenase).

NCBI and Uniprot Product Information

• NCBI GI #: 4503609
• NCBI GeneID: 2109
• NCBI Accession #: NP_001976.1
• NCBI GenBank Nucleotide #: NM_001985.2
• UniProt Accession #: P38117; Q6IBH7; Q71RF6; Q9Y3S7; A8K766; B3KNY2
• Molecular Weight: 37,434 Da
• NCBI Official Full Name: electron transfer flavoprotein subunit beta isoform 1
• NCBI Official Synonym Full Names: electron transfer flavoprotein subunit beta
• NCBI Official Symbol: ETFB
• NCBI Official Synonym Symbols: MADD; FP585
• NCBI Protein Information: electron transfer flavoprotein subunit beta
• UniProt Protein Name: Electron transfer flavoprotein subunit beta
• Protein Family: Electron transfer flavoprotein
• UniProt Gene Name: ETFB
• UniProt Synonym Gene Names: Beta-ETF

NCBI Description

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:25416781, PubMed:15159392, PubMed:15975918). It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:12815589, PubMed:7912128). ETFB binds an AMP molecule that probably has a purely structural role (PubMed:8962055, PubMed:15159392, PubMed:15975918).