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Flow Cytometry (FC/FACS) (C57BL/6 splenocytes were stained with 0.5 ug of CD160 antibody followed by F (ab'')2 Anti-Rat IgG (PE) and subsequently with CD3e antibody (FITC) and NK1.1 antibody (APC). The histogram (right) demonstrates staining of CD160 antibody on NK1.1+CD3- cells (red histogram and NK1.1+CD3+ cells (green histogram), as gated in the dot plot (left).)

Rat anti-Mouse CD160 Monoclonal Antibody | anti-CD160 antibody

Rat monoclonal CD160 antibody

Gene Names
CD160; NK1; BY55; NK28
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Functional Assay, Immunoprecipitation
Purity
Purified by affinity chromatography.
Synonyms
CD160; Monoclonal Antibody; Rat monoclonal CD160 antibody; CD160 antibody; anti-CD160 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
CNX46-3
Specificity
Mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
181
Applicable Applications for anti-CD160 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Functional Assay, Immunoprecipitation (IP)
Application Notes
FC: <= 0.5 ug/test
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10? to 10? cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(C57BL/6 splenocytes were stained with 0.5 ug of CD160 antibody followed by F (ab'')2 Anti-Rat IgG (PE) and subsequently with CD3e antibody (FITC) and NK1.1 antibody (APC). The histogram (right) demonstrates staining of CD160 antibody on NK1.1+CD3- cells (red histogram and NK1.1+CD3+ cells (green histogram), as gated in the dot plot (left).)

Flow Cytometry (FC/FACS) (C57BL/6 splenocytes were stained with 0.5 ug of CD160 antibody followed by F (ab'')2 Anti-Rat IgG (PE) and subsequently with CD3e antibody (FITC) and NK1.1 antibody (APC). The histogram (right) demonstrates staining of CD160 antibody on NK1.1+CD3- cells (red histogram and NK1.1+CD3+ cells (green histogram), as gated in the dot plot (left).)
Product Categories/Family for anti-CD160 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,810 Da
NCBI Official Full Name
CD160 antigen
NCBI Official Synonym Full Names
CD160 molecule
NCBI Official Symbol
CD160
NCBI Official Synonym Symbols
NK1; BY55; NK28
NCBI Protein Information
CD160 antigen
UniProt Protein Name
CD160 antigen
Protein Family
UniProt Gene Name
CD160
UniProt Synonym Gene Names
BY55

NCBI Description

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor showing broad specificity for both classical and non-classical MHC class I molecules.

Research Articles on CD160

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Product Notes

The CD160 cd160 (Catalog #AAA5305453) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat monoclonal CD160 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD160 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Functional Assay, Immunoprecipitation (IP). FC: <= 0.5 ug/test. Researchers should empirically determine the suitability of the CD160 cd160 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD160, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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