Mouse anti-Human CD150 Monoclonal Antibody | anti-CD150 antibody

Anti-Hu CD150 Alexa Fluor488

• Gene Names: SLAMF1; SLAM; CD150; CDw150
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD150; Monoclonal Antibody; Anti-Hu CD150 Alexa Fluor488; SLAM; SLAMF1; signaling lymphocytic activation molecule family m; anti-CD150 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: SLAM.4
• Specificity: The mouse monoclonal antibody SLAM.4 recognizes an extracellular epitope of CD150, a cell surface molecule expressed on lymphocytes and involved in their activation.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD150 antibody: Flow Cytometry (FC/FACS) (QC tested), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor488
• Immunogen: Human CD150-transfected 300.19 cells
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD150 (SLAM.4) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD150 positive lymphocytes (red-filled) from CD150 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD150 (SLAM.4) Alexa Fluor 488 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD150 antibody

CD150, also known as SLAM (signaling lymphocyte activation molecule) is a 70-95kDa single chain transmembrane phosphoglycoprotein of the CD2 family. Its extracellular part contains eight potential N-glycosylation sites, and the intracellular tail contains three unique tyrosine-based motifs. These binding sites can be recognized by SH2-binding phosphatases and the adaptor proteins, such as SAP/SH2D1A or EAT-2. The SLAM family receptors are involved in leucocyte activation and contribute to the effective germinal center formation, generation of high-affinity antibody-secreting plasma cells, and memory T and B cells, thereby facilitating long-term immune response. CD150 expression is upregulated after cell activation.

References

*Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1;174(11):7033-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/15905546 De Sal,*Romero X, Benítez D, March S, Vilella R, Miralpeix M, Engel P: Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens. 2004 Aug;64(2):132-44., URL: http://www.ncbi.nlm.nih.gov/pubmed/15245368,*De Salort J, Sintes J, Llinàs L, Matesanz-Isabel J, Engel P: Expression of SLAM (CD150) cell-surface receptors on human B-cell subsets: from pro-B to plasma cells. Immunol Lett. 2011 Jan 30;134(2):129-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/20933013,*Romero X, Martin M, Zapater N, Tovar V, Pizcueta P, Engel P: Expression of CD150 on human leukocytes. Production and characterisation of a new CD150 monoclonal antibody, URL:
*García VE, Quiroga MF, Ochoa MT, Ochoa L, Pasquinelli V, Fainboim L, Olivares LM, Valdez R, Sordelli DO, Aversa G, Modlin RL, Sieling PA: Signaling lymphocytic activation molecule expression and regulation in human intracellular infection correlate with Th1 cytokine patterns. J Immunol. 2001 Nov 15;167(10):5719-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/11698444

NCBI and Uniprot Product Information

• NCBI GI #: 9297047
• NCBI GeneID: 6504
• UniProt Accession #: Q13291; Q5W172; Q9HBE8
• Molecular Weight: 335
• NCBI Official Full Name: Signaling lymphocytic activation molecule
• NCBI Official Synonym Full Names: signaling lymphocytic activation molecule family member 1
• NCBI Official Symbol: SLAMF1
• NCBI Official Synonym Symbols: SLAM; CD150; CDw150
• NCBI Protein Information: signaling lymphocytic activation molecule; IPO-3
• UniProt Protein Name: Signaling lymphocytic activation molecule
• UniProt Gene Name: SLAMF1
• UniProt Synonym Gene Names: SLAM
• UniProt Entry Name: SLAF1_HUMAN

Uniprot Description

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; antigen binding

Biological Process: viral reproduction; regulation of catalytic activity; positive regulation of cell proliferation; regulation of vesicle fusion; lymphocyte activation; signal transduction