Mouse CD14, MONOCYTE, MACROPHAGE MARKER Monoclonal Antibody | anti-CD14 antibody
CD14, MONOCYTE, MACROPHAGE MARKER
Reactivity
Human, Porcine
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified
Synonyms
CD14; MONOCYTE; MACROPHAGE MARKER; Monoclonal Antibody; Anti -CD14; anti-CD14 antibody
Host
Mouse
Reactivity
Human, Porcine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9G141
Specificity
Recognizes human monocyte marker CD14, a 53kD glycoprotein. Recognizes both soluble and membrane CD14. Useful for inhibition of biological activity of sCD14, also inhibits LPS induced TNF production. Species crossreactivity: Porcine.
Purity/Purification
Affinity Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-CD14 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, and for inhibition of biological activity of sCD14.
Dilution: Flow Cytometry: 1:50
Dilution: Flow Cytometry: 1:50
Immunogen
Human sCD14.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CD14 antibody
CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present at low density on a subpopulation granulocytes and activated or transformed B-cells.
Product Categories/Family for anti-CD14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,667 Da
NCBI Official Full Name
CD14
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
CD14
UniProt Entry Name
CD14_BOVIN
Uniprot Description
Function: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules
Subunit structure: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4
Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor
Sequence similarities: Contains 11 LRR (leucine-rich) repeats.
Research Articles on CD14
Similar Products
Product Notes
The CD14 cd14 (Catalog #AAA608909) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD14, MONOCYTE, MACROPHAGE MARKER reacts with Human, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's CD14, MONOCYTE, MACROPHAGE MARKER can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, and for inhibition of biological activity of sCD14. Dilution: Flow Cytometry: 1:50. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, MONOCYTE, MACROPHAGE MARKER, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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